Identifying the Indyp115 Mutation of the Na+-Dicarboxylate Transporter Gene in Drosophila melanogaster

Identifying the Indyp115 Mutation of the Na+-Dicarboxylate Transporter Gene in Drosophila... The P[lArB]-element insertional mutation (Indy p115) was obtained in the promoter region of the Na+-dicarboxylate transporter gene of Drosophila (geneIndy, I'm not dead yet) within the 75D region of chromosome 3. The expression pattern of the reporter β-galactosidase was determined in various tissues of third-instar larvae and adult flies. Both males and females homozygous for this mutation were fertile, though their viability was reduced. The two lethality phases were revealed in embryogenesis at nucleus cleavage stages 1–5 to the point of blastoderm formation and at latest stages 15–16, as well as at stage 17, when the larval development is completed though the larva still remains in the chorion. The gene expression in the follicular cells of an embryo at the terminal oogenesis stages is suggested to cause the first lethality stage. The expression pattern of this gene seems also to account for the tissue- and stage-specific activity of the 5"-regulatory region in the Indy gene. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Identifying the Indyp115 Mutation of the Na+-Dicarboxylate Transporter Gene in Drosophila melanogaster

Loading next page...
 
/lp/springer_journal/identifying-the-indyp115-mutation-of-the-na-dicarboxylate-transporter-KY3GAt4ouD
Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1013707610019
Publisher site
See Article on Publisher Site

Abstract

The P[lArB]-element insertional mutation (Indy p115) was obtained in the promoter region of the Na+-dicarboxylate transporter gene of Drosophila (geneIndy, I'm not dead yet) within the 75D region of chromosome 3. The expression pattern of the reporter β-galactosidase was determined in various tissues of third-instar larvae and adult flies. Both males and females homozygous for this mutation were fertile, though their viability was reduced. The two lethality phases were revealed in embryogenesis at nucleus cleavage stages 1–5 to the point of blastoderm formation and at latest stages 15–16, as well as at stage 17, when the larval development is completed though the larva still remains in the chorion. The gene expression in the follicular cells of an embryo at the terminal oogenesis stages is suggested to cause the first lethality stage. The expression pattern of this gene seems also to account for the tissue- and stage-specific activity of the 5"-regulatory region in the Indy gene.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 13, 2004

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from Google Scholar, PubMed
Create lists to organize your research
Export lists, citations
Access to DeepDyve database
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off