Identification of Wheat d-Cysteine Desulfhydrase (TaD-CDes) Required for Abscisic Acid Regulation of Seed Germination, Root Growth, and Stomatal Closure in Arabidopsis

Identification of Wheat d-Cysteine Desulfhydrase (TaD-CDes) Required for Abscisic Acid Regulation... Cysteine desulfhydrase (CDes) can catalyze the degradation of cysteine producing hydrogen sulfide. In this study, d -cysteine desulfhydrase from wheat (TaD-CDes) was cloned and overexpressed in Arabidopsis thaliana. The physiological effects of TaD-CDes were determined by investigating seed germination, root growth, stomatal closure, and drought resistance in the TaD-CDes plants. Results showed that, compared with wild-type plants (WT), seed germination, root growth, and stomatal closure of the TaD-CDes plants were more sensitive to ABA, resulting from up-regulation of ABA-responsive genes (such as PYR1, ABI1, ABI2, HAB1, HAB2, SnRK2, ABF2, and ABF4). Moreover, although TaD-CDes mediated ABA-induced stomatal closure, TaD-CDes-overexpressing plants did not show higher drought resistance than WT, which might be attrib- uted to their increased stomatal densities. Keywords d -Cysteine desulfhydrase · Germination · Root · Stomata · ABA Introduction the third gaseous signal molecule in plants after nitric oxide (NO) and carbon monoxide (CO). Hydrogen sulfide (H S) has been studied for over 300 years. Cysteine desulfhydrase (CDes) can catalyze the degra- For a long time, it was thought to be toxic, as high concentra- dation of cysteine into H S, pyruvate and NH , and is the 2 3 tions of H S inhibit the activity of mitochondrial cytochrome main source http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Plant Growth Regulation Springer Journals

Identification of Wheat d-Cysteine Desulfhydrase (TaD-CDes) Required for Abscisic Acid Regulation of Seed Germination, Root Growth, and Stomatal Closure in Arabidopsis

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Publisher
Springer US
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Life Sciences; Plant Sciences; Plant Anatomy/Development; Plant Physiology; Agriculture
ISSN
0721-7595
eISSN
1435-8107
D.O.I.
10.1007/s00344-018-9817-8
Publisher site
See Article on Publisher Site

Abstract

Cysteine desulfhydrase (CDes) can catalyze the degradation of cysteine producing hydrogen sulfide. In this study, d -cysteine desulfhydrase from wheat (TaD-CDes) was cloned and overexpressed in Arabidopsis thaliana. The physiological effects of TaD-CDes were determined by investigating seed germination, root growth, stomatal closure, and drought resistance in the TaD-CDes plants. Results showed that, compared with wild-type plants (WT), seed germination, root growth, and stomatal closure of the TaD-CDes plants were more sensitive to ABA, resulting from up-regulation of ABA-responsive genes (such as PYR1, ABI1, ABI2, HAB1, HAB2, SnRK2, ABF2, and ABF4). Moreover, although TaD-CDes mediated ABA-induced stomatal closure, TaD-CDes-overexpressing plants did not show higher drought resistance than WT, which might be attrib- uted to their increased stomatal densities. Keywords d -Cysteine desulfhydrase · Germination · Root · Stomata · ABA Introduction the third gaseous signal molecule in plants after nitric oxide (NO) and carbon monoxide (CO). Hydrogen sulfide (H S) has been studied for over 300 years. Cysteine desulfhydrase (CDes) can catalyze the degra- For a long time, it was thought to be toxic, as high concentra- dation of cysteine into H S, pyruvate and NH , and is the 2 3 tions of H S inhibit the activity of mitochondrial cytochrome main source

Journal

Journal of Plant Growth RegulationSpringer Journals

Published: May 30, 2018

References

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