Identification of the essential and non-essential transcription units for protein synthesis, DNA replication and infectious virus production of Porcine circovirus type 1

Identification of the essential and non-essential transcription units for protein synthesis, DNA... A plasmid-based transfection system capable of yielding infectious Porcine circovirus type 1 (PCV1) was established and mutational analysis was conducted to investigate the involvement of each viral transcription unit in protein synthesis, DNA replication and progeny virus production. During PCV1 replication in PK15 cells, twelve viral-specific RNAs are synthesized. They include the capsid protein RNA ( CR ), eight Rep-associated RNAs ( Rep , Rep ’, Rep3a , Rep3b , Rep3c-1 , Rep3c-2 , Rep3c-3 and Rep3c-4 ), and three NS-associated RNAs ( NS462 , NS642 and NS0 ). A stop codon introduced at the 5’-end of CR did not affect Rep-associated antigens or viral DNA synthesis. Altering the consensus dinucleotide at the splice junctions of the Rep3 RNAs and NS462 or introducing an early termination codon in Rep3c-4 and NS0 also did not have any affect on virus replication. However, mutations in Rep and Rep ’ caused greater than 99% reduction of protein synthesis and complete shut down of viral DNA replication. NS642 could not be assayed in this study because silent mutation at the splice junction was not possible. However, it is probably equivalent to the non-essential RNA ( NS672 ) of PCV type 2. Thus, only two proteins, Rep and Rep’, are essential for PCV1 protein, DNA and infectious virus biosynthesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Identification of the essential and non-essential transcription units for protein synthesis, DNA replication and infectious virus production of Porcine circovirus type 1

Loading next page...
 
/lp/springer_journal/identification-of-the-essential-and-non-essential-transcription-units-dGS0CzNUbI
Publisher
Springer Journals
Copyright
Copyright © 2004 by Springer-Verlag/Wien
Subject
LifeSciences
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-003-0249-8
Publisher site
See Article on Publisher Site

Abstract

A plasmid-based transfection system capable of yielding infectious Porcine circovirus type 1 (PCV1) was established and mutational analysis was conducted to investigate the involvement of each viral transcription unit in protein synthesis, DNA replication and progeny virus production. During PCV1 replication in PK15 cells, twelve viral-specific RNAs are synthesized. They include the capsid protein RNA ( CR ), eight Rep-associated RNAs ( Rep , Rep ’, Rep3a , Rep3b , Rep3c-1 , Rep3c-2 , Rep3c-3 and Rep3c-4 ), and three NS-associated RNAs ( NS462 , NS642 and NS0 ). A stop codon introduced at the 5’-end of CR did not affect Rep-associated antigens or viral DNA synthesis. Altering the consensus dinucleotide at the splice junctions of the Rep3 RNAs and NS462 or introducing an early termination codon in Rep3c-4 and NS0 also did not have any affect on virus replication. However, mutations in Rep and Rep ’ caused greater than 99% reduction of protein synthesis and complete shut down of viral DNA replication. NS642 could not be assayed in this study because silent mutation at the splice junction was not possible. However, it is probably equivalent to the non-essential RNA ( NS672 ) of PCV type 2. Thus, only two proteins, Rep and Rep’, are essential for PCV1 protein, DNA and infectious virus biosynthesis.

Journal

Archives of VirologySpringer Journals

Published: May 1, 2004

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off