Identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China

Identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China Three virus isolates from maize with rough dwarf in different provinces in China were analyzed at the molecular level. When compared to an isolate from diseased rice plants in Hubei Province, all four isolates had identical genomic RNA electrophoretic profiles, which were composed of ten double-stranded (ds) RNAs. Full-length cDNAs of segment 10 (S10) from each of the four isolates were cloned by RT-PCR and the complete sequences were determined. Analysis of the sequences revealed that each consisted of 1801 nucleotides and contained a single open reading frame (ORF) which potentially encoded a protein with 558 amino acids. Further, the sequences showed more than 97.0% and 98.0% identity atnucleotide and amino acid levels, respectively. In addition, theiridentities to rice black-streaked dwarf virus S10 were significantlyhigher than those to maize rough dwarf virus S10. Based on theseresults, it is suggested that the virus which causes this maize diseasein China is rice black-streaked dwarf virus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China

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Publisher
Springer Journals
Copyright
Copyright © 2001 by Springer-Verlag/Wien
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050170200
Publisher site
See Article on Publisher Site

Abstract

Three virus isolates from maize with rough dwarf in different provinces in China were analyzed at the molecular level. When compared to an isolate from diseased rice plants in Hubei Province, all four isolates had identical genomic RNA electrophoretic profiles, which were composed of ten double-stranded (ds) RNAs. Full-length cDNAs of segment 10 (S10) from each of the four isolates were cloned by RT-PCR and the complete sequences were determined. Analysis of the sequences revealed that each consisted of 1801 nucleotides and contained a single open reading frame (ORF) which potentially encoded a protein with 558 amino acids. Further, the sequences showed more than 97.0% and 98.0% identity atnucleotide and amino acid levels, respectively. In addition, theiridentities to rice black-streaked dwarf virus S10 were significantlyhigher than those to maize rough dwarf virus S10. Based on theseresults, it is suggested that the virus which causes this maize diseasein China is rice black-streaked dwarf virus.

Journal

Archives of VirologySpringer Journals

Published: Jan 1, 2001

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