Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine

Identification of QTL affecting resistance/susceptibility to acute Actinobacillus... Actinobacillus pleuropneumoniae is among the most important pathogens worldwide in pig production. The agent can cause severe economic losses due to decreased performance, acute or chronic pleuropneumonia and an increased incidence of death. Therapeutics cannot be used in a sustainable manner, and vaccination is not always available, but discovering more about host defence and disease mechanisms might lead to new methods of prophylaxis. The aim of the present study was to detect quantitative trait loci (QTL) associated with resistance/susceptibility to A. pleuropneumoniae. Under controlled conditions, 170 F2 animals of a Hampshire/Landrace family, with known differences in founder populations regarding A. pleuropneumoniae resistance, were challenged with an A. pleuropneumoniae serotype 7 aerosol followed by a detailed clinical, radiographic, ultrasonographic, pathological and bacteriological examination. F2 pigs were genotyped with 159 microsatellite markers. Significant QTL were identified on Sus scrofa chromosomes (SSC) 2, 6, 12, 13, 16, 17 and 18. They explained 6–22 % of phenotypic variance. One QTL on SSC2 reached significance on a genome-wide level for five associated phenotypic traits. A multiple regression analysis revealed a combinatory effect of markers SWR345 (SSC2) and S0143 (SSC12) on Respiratory Health Score, Clinical Score and the occurrence of death. The results indicate the genetic background of A. pleuropneumoniae resistance in swine and provide new insights into the genetic architecture of resistance/susceptibility to porcine pleuropneumonia. The results will be helpful in identifying the underlying genes and mechanisms. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer Science+Business Media New York
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
DOI
10.1007/s00335-013-9497-4
pmid
24445419
Publisher site
See Article on Publisher Site

Abstract

Actinobacillus pleuropneumoniae is among the most important pathogens worldwide in pig production. The agent can cause severe economic losses due to decreased performance, acute or chronic pleuropneumonia and an increased incidence of death. Therapeutics cannot be used in a sustainable manner, and vaccination is not always available, but discovering more about host defence and disease mechanisms might lead to new methods of prophylaxis. The aim of the present study was to detect quantitative trait loci (QTL) associated with resistance/susceptibility to A. pleuropneumoniae. Under controlled conditions, 170 F2 animals of a Hampshire/Landrace family, with known differences in founder populations regarding A. pleuropneumoniae resistance, were challenged with an A. pleuropneumoniae serotype 7 aerosol followed by a detailed clinical, radiographic, ultrasonographic, pathological and bacteriological examination. F2 pigs were genotyped with 159 microsatellite markers. Significant QTL were identified on Sus scrofa chromosomes (SSC) 2, 6, 12, 13, 16, 17 and 18. They explained 6–22 % of phenotypic variance. One QTL on SSC2 reached significance on a genome-wide level for five associated phenotypic traits. A multiple regression analysis revealed a combinatory effect of markers SWR345 (SSC2) and S0143 (SSC12) on Respiratory Health Score, Clinical Score and the occurrence of death. The results indicate the genetic background of A. pleuropneumoniae resistance in swine and provide new insights into the genetic architecture of resistance/susceptibility to porcine pleuropneumonia. The results will be helpful in identifying the underlying genes and mechanisms.

Journal

Mammalian Genome – Springer Journals

Published: Jan 21, 2014

References

  • Sex-specific effect of IL9 polymorphisms on lung function and polysensitization
  • Actinobacillus pleuropneumoniae iron transport and urease activity: effects on bacterial virulence and host immune response
    Baltes, N; Topitak, W; Gerlach, GF; Hennig-Pauka, I; Hoffmann-Moujahid, M; Ganter, M; Rothkotter, HJ
  • Identification of dimethyl sulfoxide reductase in Actinobacillus pleuropneumoniae and its role in infection
    Baltes, N; Hennig-Pauka, I; Jacobsen, I; Gruber, AD; Gerlach, GF
  • Actinobacillus pleuropneumoniae serotype 7 siderophore receptor FhuA is not required for virulence
    Baltes, N; Tonpitak, W; Hennig-Pauka, I; Gruber, AD; Gerlach, GF
  • A major QTL associated with host response to porcine reproductive and respiratory syndrome virus challenge
    Boddicker, N; Rowland, R; Lunney, JK; Garrick, DJ; Reecy, J; Dekkers, JCM
  • Inborn errors of human JAKs and STATs
    Casanova, JL; Holland, SM; Notarangelo, LD
  • Virulence factors of Actinobacillus pleuropneumoniae involved in colonization, persistence and induction of lesions in its porcine host
    Chiers, K; Waele, T; Pasmans, F; Ducatelle, R; Hasebrouck, F
  • Expression of mRNA encoding interleukin (IL)-10, IL-12p35 and IL-12p40 in lungs from pigs experimentally infected with Actinobacillus pleuropneumoniae
    Cho, WS; Jung, K; Kim, J; Ha, Y; Chae, C
  • Empirical threshold values for quantitative trait mapping
    Churchill, GA; Doerge, RW
  • Chronic pleuritis in Danish slaughter pig herds
    Cleveland-Nielsen, A; Nielsen, EO; Ersbol, AK
  • Use of recombinant ApxIV in serodiagnosis of Actinobacillus pleuropneumoniae infections, development and prevalidation of the Apx IV Elisa
    Dreyfus, A; Schaller, A; Nivollet, S; Segers, RPAM; Kobisch, M; Mieli, L; Soerensen, V; Hussy, D; Miserez, R; Zimmermann, W; Inderbitzin, F; Frey, J
  • Mapping quantitative trait loci for immune capacity in the pig
    Edfors-Lilja, I; Wattrang, E; Marklund, L; Moller, M; Andersson-Eklund, L; Andersson, L; Fossum, C
  • Porcine pleuropneumonia
    Fenwick, B; Henry, S
  • Diseases of swine
    Gottschalk, M; Taylor, DJ
  • Documentation for CRI-MAP, version 2.4
    Green, P; Falls, K; Crooks, S
  • Identification of QTL for dorso-caudal chronic pleuritis in 12 crossbred porcine families
    Gregersen, VR; Sørensen, KK; Christensen, OF; Busch, ME; Vingborg, RKK; Velander, IH; Lund, MS; Bendixen, C
  • Changes in antimicrobial susceptibility of Actinobacillus pleuropneumoniae isolated from pigs in Spain during the last decade
    Gutiérrez-Martín, CB; García Del Blanco, N; Blanco, M; Navas, J; Rodríguez-Ferri, EF
  • Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection
    Haesebrouck, F; Chiers, K; Overbeke, I; Ducatelle, R
  • Genome mapping
    Haley, CS; Andersson, L
  • Mapping quantitative trait loci in crosses between outbred lines using least squares
    Haley, CS; Knott, SA; Elsen, J-M
  • Tylosin tartrate and tiamutilin effects on experimental piglet pneumonia induced with pig lung homogenate containing mycoplasmas, bacteria and viruses
    Hannan, PC; Bhogal, BS; Fish, JP
  • Evaluation of a killed vaccine against porcine pleuropneumoniae due to Haemophilus pleuropneumoniae
    Higgins, R; Lariviere, S; Mittal, KR; Martineau, GP; Rousseau, P; Cameron, J
  • A novel Respiratory Health Score (RHS) supports a role of acute lung damage and pig breed in the course of an Actinobacillus pleuropneumoniae infection
    Hoeltig, D; Hennig-Pauka, I; Thies, K; Rehm, T; Beyerbach, M; Strutzberg-Minder, K; Gerlach, GF; Waldmann, KH
  • A genome scan for quantitative trait loci and imprinted regions affecting reproduction in pigs
    Holl, JW; Cassady, JP; Johnson, RK
  • Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae
    Jacobsen, MJ; Nielsen, JP
  • Comparison of virulence of different Actinobacillus pleuropneumoniae serotypes and biotypes using an aerosol infection model
    Jacobsen, MJ; Nielsen, JP; Nielsen, R
  • Enzymes involved in anaerobic respiration appear to play a role in Actinobacillus pleuropneumoniae virulence
    Jacobsen, I; Hennig-Pauka, I; Baltes, N; Trost, M; Gerlach, GF
  • Pleuritis in slaughter pigs: relations between lung lesions and bacteriology in 10 herds with high pleuritis
    Jirawattanapong, P; Stockhofe-Zurwieden, N; Leengoed, L; Wisselink, H; Raymakers, R; Cruijsen, T; Peet-Schwering, C; Nielen, M; Nes, A
  • A novel enzymelinked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds
    Leiner, G; Franz, B; Strutzberg, K; Gerlach, GF
  • Genome-wide association study for T lymphocyte subpopulations in swine
    Lu, X; Fu, WX; Luo, YR; Ding, XD; Zhou, JP; Liu, Y; Liu, JF; Zhang, Q
  • Probing genetic control of swine responses to PRRSV infection: current progress of the PRRS host genetics consortium
    Lunney, JK; Steibel, JP; Reecy, JM; Fritz, E; Rothschild, MF; Kerrigan, M; Trible, B; Rowland, RRR
  • Development of a DIVA subjunit vaccine against Actinobacillus pleuropneumoniae infection
    Maas, A; Meens, J; Baltes, N; Hennig-Pauka, I; Gerlach, GF
  • Comparison of the effect of pneumonia detected during lifetime with pneumonia detected at slaughter on growth in swine
    Noyes, EP; Feeney, D; Pijoan, C
  • Design of a high density SNP genotyping assay in the pig using SNPs identified and characterized by next generation sequencing technology
    Ramos, AM; Crooijmans, RPMA; Affara, NA; Amaral, AJ; Archibald, AL
  • Investigations on genetic disease resistance in swine—a contribution to the reduction of pain, suffering and damage in farm animals
    Reiner, G
  • Detection of quantitative trait loci for resistance/susceptibility to the pseudorabies virus in swine
    Reiner, G; Melchinger, E; Kramarova, M; Pfaff, E; Büttner, M; Saalmüller, A; Geldermann, H
  • Mapping of quantitative trait loci affecting resistance/susceptibility to Sarcocystis miescheriana in swine
    Reiner, G; Kliemt, D; Willems, H; Berge, T; Fischer, R; Köhler, F; Hepp, S; Hertrampf, B; Daugschies, A; Geldermann, H; Mackenstedt, U; Zahner, H
  • Quantitative trait loci for white blood cell numbers in swine
    Reiner, G; Fischer, R; Hepp, S; Berge, T; Köhler, F; Willems, H
  • Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice
    Shao, M; Wang, Y; Wang, C; Guo, Y; Peng, Y; Liu, J; Li, G; Liu, H; Liu, S
  • Field experience with two different vaccination strategies aiming to control infections with Actinobacillus pleuropneumoniae in a fattening pig herd
    Sjölund, S; Wallgren, P
  • Genomic regions associated with ventro-cranial chronic pleuritis in pig
    Sorensen, KK; Gregersen, VR; Christensen, OF; Velander, IH; Bendixen, C
  • Bridging the genotyping gap: using genotyping by sequencing (GBS) to add high-density SNP markers and new value to traditional bi-parental mapping and breeding populations
    Spindel, J; Wright, M; Chen, C; Cobb, J; Gage, J; Harrington, S; Lorieux, M; Ahmadi, N; McCouch, S
  • Construction of an Actinobacillus pleuropneumoniae serotype 2 prototype live negative-marker vaccine
    Tonpitak, W; Baltes, N; Hennig-Pauka, I; Gerlach, GF
  • Impaired CD14 and CD36 expression, bacterial clearance, and Toll-like receptor 4YMYD88 signaling in Caveolin-1Y deleted macrophages and mice
    Tsai, TH; Chen, SF; Huang, TY; Tzeng, CF; Chiang, AS; Kou, YR; Lee, TS; Shyue, SK
  • Comparison of the efficacy of a subunit and a live streptomycin-dependent porcine pleuropneumonia vaccine
    Tumamao, JQ; Bowles, RE; Bosch, H; Klaasen, HL; Fenwick, BW; Storie, GJ; Blackall, PJ
  • Mapping quantitative trait loci for innate immune response in the pig
    Uddin, MJ; Cinar, MU; Große-Brinkhaus, C; Tesfaye, D; Tholen, E; Juengst, H; Looft, C; Wimmers, K; Phatsara, C; Schellander, K
  • Vaccination in food animal populations
    Oirschot, JT
  • Antimicrobial resistance of food born pathogens
    White, DG; Zhao, S; Simjee, S; Wagner, DD; McDermott, PF
  • Verification of chromosomal regions affecting the innate immunity in pigs using linkage mapping
    Wimmers, K; Jonas, E; Schreinemachers, HJ; Tesfaye, D; Ponsuksili, S; Tholen, E; Juengst, H; Schellander, K; Phatsara, C
  • QTL for traits related to humoral immune response estimated from data of a porcine F2 resource population
    Wimmers, K; Murani, E; Schellander, K; Ponsuksili, S

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