Identification of novel E6-E7 sequence variants of human papillomavirus 16

Identification of novel E6-E7 sequence variants of human papillomavirus 16 The rate of evolution of the human papillomavirus 16 (HPV16) genome is low. However, the ability of the E6 oncoprotein to interact with distinct p53 variants causes selective pressure on the E6 gene. In addition, intratypic recombination events in the HPV16 E6 and E7 genes have been characterized as extraordinary phenomena during the evolutionary history of virus. In the present study, we identified two new sequence variants through nucleotide analysis of the E6-E7 region of the HPV16 genome. Maximum-likelihood and empirical Bayesian methods were used in order to identify positive selection at particular residues of the E6 and E7 genes. Using the single recombination breakpoint (SBP) method, we found evidence of recombination events in the E6 ORF. Nucleotide sequence analysis showed that the new sequence variants are phylogenetically distant from the other members of the population. Our results indicate that new evolutionary intermediates of HPV16 might be formed either though positive selective pressure or through recombination events by multiple infections with distinct HPV16 variants. Archives of Virology Springer Journals

Identification of novel E6-E7 sequence variants of human papillomavirus 16

Loading next page...
Springer Vienna
Copyright © 2013 by Springer-Verlag Wien
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
Publisher site
See Article on Publisher Site


  • Human papillomavirus type 16 E2 and E6/E7 variants
    Swan, DC; Rajeevan, M; Tortolero-Luna, G; Follen, M; Tucker, RA; Unger, ER
  • New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay
    Casas, I; Powell, L; Klapper, PE; Cleator, GM
  • Datamonkey: rapid detection of selective pressure on individual sites of codon alignments
    Pond, SL; Frost, SD
  • Human papillomavirus 16 E6, L1, L2 and E2 gene variants in cervical lesion progression
    Lee, K; Magalhaes, I; Clavel, C; Briolat, J; Birembaut, P; Tommasino, M; Zehbe, I
  • Molecular and phylogenetic analysis of the HPV 16 E4 gene in cervical lesions from women in Greece
    Tsakogiannis, D; Ruether, IG; Kyriakopoulou, Z; Pliaka, V; Skordas, V; Gartzonika, C; Levidiotou-Stefanou, S; Markoulatos, P
  • Phylogenetics by likelihood: evolutionary modeling as a tool for understanding the genome
    Kosiol, C; Bofkin, L; Whelan, S
  • Codon-substitution models for heterogeneous selection pressure at amino acid sites
    Yang, Z; Nielsen, R; Goldman, N; Pedersen, AM

You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial