Identification of new early auxin markers in tobacco by mRNA differential display

Identification of new early auxin markers in tobacco by mRNA differential display Differential display of mRNA has been improved by developing a two-step PCR amplification procedure. The modified differential display has been applied to identify early alterations of mRNA expression in response to auxin treatment of tobacco seedlings. This approach has led to the isolation of four fragments corresponding to auxin-up-regulated mRNAs. One, named GO15-13, shows significant homology with the 3′ end of the coding region of the soybean SAUR X10A [10]. The three other fragments present no homology with sequences available in the databases and constitute potential new early auxin markers. Plant Molecular Biology Springer Journals

Identification of new early auxin markers in tobacco by mRNA differential display

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Kluwer Academic Publishers
Copyright © 1998 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
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