Identification of lymphocystis disease virus from paradise fish Macropodus opercularis (LCDV-PF)

Identification of lymphocystis disease virus from paradise fish Macropodus opercularis (LCDV-PF) Iridoviruses are large DNA viruses that are subdivided into five genera: Ranavirus , Megalocytivirus , Lymphocystivirus , Chloriridovirus and Iridovirus . The iridovirus lymphocystis disease virus (LCDV) is an important fish pathogen that can infect marine and freshwater fish worldwide. In this study, we have identified the pathogen in paradise fish ( Macropodus opercularis ) with lymphocystis. On the skin and fins of diseased paradise fish, a large number of nodules were observed. H&E staining showed that the nodules were composed of encapsulated hypertrophied cells. Using electron microscopy, numerous virus particles with a diameter of >210 nm and with hexagonal profiles were observed in the cytoplasm. Phylogenetic analysis based on the major capsid protein (MCP), DNA polymerase and myristylated membrane protein (MMP) genes revealed that LCDV from paradise fish (LCDV-PF) was closely related to lymphocystis disease virus from China (LCDV-C), followed by lymphocystis disease virus 1 (LCDV-1). Taken together, our data provide the first molecular evidence that, in addition to megalocytivirus, LCDV is an important iridoviral pathogen in paradise fish besides megalocytivirus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Identification of lymphocystis disease virus from paradise fish Macropodus opercularis (LCDV-PF)

Loading next page...
 
/lp/springer_journal/identification-of-lymphocystis-disease-virus-from-paradise-fish-1Lglt5aBAw
Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2060-0
Publisher site
See Article on Publisher Site

Abstract

Iridoviruses are large DNA viruses that are subdivided into five genera: Ranavirus , Megalocytivirus , Lymphocystivirus , Chloriridovirus and Iridovirus . The iridovirus lymphocystis disease virus (LCDV) is an important fish pathogen that can infect marine and freshwater fish worldwide. In this study, we have identified the pathogen in paradise fish ( Macropodus opercularis ) with lymphocystis. On the skin and fins of diseased paradise fish, a large number of nodules were observed. H&E staining showed that the nodules were composed of encapsulated hypertrophied cells. Using electron microscopy, numerous virus particles with a diameter of >210 nm and with hexagonal profiles were observed in the cytoplasm. Phylogenetic analysis based on the major capsid protein (MCP), DNA polymerase and myristylated membrane protein (MMP) genes revealed that LCDV from paradise fish (LCDV-PF) was closely related to lymphocystis disease virus from China (LCDV-C), followed by lymphocystis disease virus 1 (LCDV-1). Taken together, our data provide the first molecular evidence that, in addition to megalocytivirus, LCDV is an important iridoviral pathogen in paradise fish besides megalocytivirus.

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 2014

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off