1022-7954/05/4112- © 2005 Pleiades Publishing, Inc.
Russian Journal of Genetics, Vol. 41, No. 12, 2005, pp. 1372–1380. From Genetika, Vol. 41, No. 12, 2005, pp. 1656–1664.
Original English Text Copyright © 2005 by Shang, Wei, Long, Yan, Zheng.
has been recognized [6–8].
The genus Secale L. (Triticeae; Poaceae), the tertia-
ry gene pool of wheat , has shown great potential to
be a valuable genetic resource to increase the genetic
variability and to introduce desirable genes/characters
for wheat improvement. To date, considerable progress
has been achieved in biochemical extraction and char-
acterization of the prolamin fractions (secalin) in culti-
vated rye [10–22]. SDS-PAGE analysis revealed that
the prolamin fractions of rye contained four storage
protein types (i.e., HMW, γ–75k, γ–40k and ω-secalins)
, while three types
of wheat storage proteins (i.e.,
α/β-gliadins and LMW glutenins) could not be identi-
fied in rye .
According to the taxonomic systems in the two genetic
resource networks GRIN (http://www.ars-grin.gov/) and
PGRC (http://pgrc3.agr.gc.ca/), the genus Secale was
commonly recognized as four species, one annual out-
breeding species (i.e., S. cereale), two annual inbreed-
ing species (i.e., S. vavilovii and S. sylvestre) and one
perennial outbreeding (i.e., S. strictum) species. Sever-
al subspecies were recognized in S. cereale and S. stric-
tum, among which S. cereale ssp. cereale was the one
and only cultivated
rye. Most of them are difficult or
even impossible to distinguish morphologically except
S. sylvestre, which is clearly morphologically distinct
from all other taxa and has been confirmed to be well
diverged from other species [14, 23–27]. Due to its par-
ticular morphological characters and the highly autog-
amous habit, S. sylvestre had been selected as
able material in evolutionary studies of the R genome
of Secale .
The objective of this paper is to identify and isolate
the LMW glutenin-like genes in the R genome of Se-
cale, and to obtain some valuable information for fur-
ther investigation of the storage proteins in Secale.
Plant material. Nineteen Secale accessions, includ-
ing 4 species and 10 subspecies, were analyzed (Table 1).
NGB5073 was obtained from Nordic Gene Bank, Swe-
den. CN31389 was kindly provided by Dr. D. Kessler
in Plant Gene Resources of Canada (PGRC). R953/90
and R955/90 were kindly provided by Dr. A. Graner in
Identification of LMW Glutenin-Like Genes
from Secale sylvestre Host*
H.-Y. Shang, Y.-M. Wei, H. Long, Z.-H. Yan, and Y.-L. Zheng
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan, 611830 China;
fax: 86-835-288-31-53; e-mail: firstname.lastname@example.org
Received April 7, 2005
Abstract—Three low-molecular-weight (LMW) glutenin-like genes (designated as Ssy1, Ssy2, and Ssy3) from
Secale sylvestre Host were isolated and characterized. The three genes consist of a predicted highly conserva-
tive signal peptide with 20 amino acids, a short N-terminal region with 13 amino acids, a highly variable repet-
itive domain and a less variable C-terminal domain. The deduced amino acid sequences of the three genes were
the LMW-m type due to a methionine residue at the N-terminus. The phylogenetic analysis indicated that the
prolamin genes could be perfectly clustered into ﬁve groups, including HMW-GS, LMW-GS, α/β-, γ-, and
ω-prolamin. The LMW glutenin-like genes of S. sylvestre were more orthologous with the LMW-GS genes of
wheat and B hordein genes of barley, which also had been conﬁrmed by the homology analysis with the LMW-GS
of wheat at Glu-A3, Glu-B3, and Glu-D3 loci. These results indicated that a chromosome locus (designated as
Glu-R3) might be located on the R genome of S. sylvestre with the functions similar to the Glu-3 locus in wheat
and its related species.
* The text was submitted by the authors in English.