ISSN 10227954, Russian Journal of Genetics, 2014, Vol. 50, No. 7, pp. 757–762. © Pleiades Publishing, Inc., 2014.
Original Russian Text © D.A. Plakhina, E.U. Zvychaynaya, M.V. Kholodova, A.A. Danilkin, 2014, published in Genetika, 2014, Vol. 50, No. 7, pp. 862–867.
At present, the European (
) roe deer reside in Eastern
Europe. The “hybridization zone,” which formed as a
result of natural and artificial animal settling, extends
and spreads from the right side of the Volga River up to
Eastern Poland [1–3; Matosiuk, M., Borkowska, A.,
and Swislocka, M., unpublished data]. For example,
22% of animals in the Moscow region carry the
mtDNA of the European roe deer and 78% of the
Siberian . Zoologists, systematicians, and hunters
often have problems with the identification of animal
species and trophies because of interspecies hybridiza
tion . It is likely that the a high number of the many
trophies of European roe deer obtained in Eastern
Europe (Table 1) in fact belong to Siberian roe deer or
may belong to hybrid animals.
Identification of the European and the Siberian roe
deer on the spot is possible according to several mor
phologic traits [7, 8]; however, in many cases (specifi
cally in the trophy business) they are not applicable. In
the last two decades, the exact identification of roe
deer species is conducted using molecular and genetic
methods [3, 5, 6, 9–12].
Identification of European (
L.) and Siberian
Pall.) Roe Deer Hybrids by Microsatellite
D. A. Plakhina, E. Yu. Zvychaynaya, M. V. Kholodova, and A. A. Danilkin
Severtsov Institute of Ecology and Evolution Problems, Russian Academy of Sciences, Moscow, 119071 Russia
Received December 16, 2013; in final form February 3, 2014
—An analysis of 130 tissue specimens of the European (
) and the Siberian
) roe deer from nine regions of Russia and Ukraine using microsatellite loci analysis was con
ducted, aimed at the identification of hybrid animals. An optimized complex of 21 microsatellite loci was
selected for the determination of species specificity and the search for interspecies hybrids. Hybrid animals
were revealed in the Moscow and Volgograd regions. Their ratio in the total sample obtained in the European
part of Russia was 11.9%.
Recorded parameters of trophy of European and Siberian roe deer obtained in Russia at the end of 20th–beginning
of 21st centuries*
Prey location, region Antler length, cm Antlerspan, cm
European roe deer
Moscow** 31.8/28.9 27.6 12.4/12.7 3/3
** 24.8/27.3 22.9 11.4/11.4 3/3
Moscow 25.1/25.7 16.2 9.5/10.2 3/3
Belgorodskaya 25.1/26.4 11.7 12.4/13.3 3/3
Siberian roe deer
Kurganskaya 40.3/42.2 47.3 18.4/18.1 3/3
34.3/35.2 39.1 17.1/17.8 3/3
37.5/35.2 35.6 13.3/13.0 3/3
32.4/31.1 26.0 21.9/21.9 5/5
34.9/36.5 27.3 16.8/17.1 5/3
? 32.7/32.1 31.8 16.5/18.1 6/7
* According to .
** Presumable trophies of Siberian roe deer or hybrid animals.