Identification of (-)-epigallocatechin-3-gallate as a potential agent for blocking infection by grass carp reovirus

Identification of (-)-epigallocatechin-3-gallate as a potential agent for blocking infection by... Grass carp reovirus (GCRV), the representative strain of the species Aquareovirus C , serves as a model for studying the pathogenesis of aquareoviruses. Previously, epigallocatechin gallate (EGCG) was shown to inhibit orthoreovirus infection. The aim of this study was to test its potential in blocking infection by GCRV. We show that adhesion to the CIK ( Ctenopharyngodon idellus kidney) cell surface by GCRV particles is inhibited in a dose-dependent manner by EGCG, as well as by a crude extract of green tea. We also evaluated the safety of EGCG and green tea extract using CIK cells, and the results suggest that EGCG is a promising compound that may be developed as a plant-derived small molecular therapeutic agent against grass carp hemorrhagic disease caused by GCRV infection. As the ligand for the 37/67-kDa laminin receptor (LamR), EGCG’s blocking effect on GCRV attachment was associated with the binding potential of GCRV particles to LamR, which was inferred from a VOPBA assay. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Identification of (-)-epigallocatechin-3-gallate as a potential agent for blocking infection by grass carp reovirus

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-016-2751-9
Publisher site
See Article on Publisher Site

Abstract

Grass carp reovirus (GCRV), the representative strain of the species Aquareovirus C , serves as a model for studying the pathogenesis of aquareoviruses. Previously, epigallocatechin gallate (EGCG) was shown to inhibit orthoreovirus infection. The aim of this study was to test its potential in blocking infection by GCRV. We show that adhesion to the CIK ( Ctenopharyngodon idellus kidney) cell surface by GCRV particles is inhibited in a dose-dependent manner by EGCG, as well as by a crude extract of green tea. We also evaluated the safety of EGCG and green tea extract using CIK cells, and the results suggest that EGCG is a promising compound that may be developed as a plant-derived small molecular therapeutic agent against grass carp hemorrhagic disease caused by GCRV infection. As the ligand for the 37/67-kDa laminin receptor (LamR), EGCG’s blocking effect on GCRV attachment was associated with the binding potential of GCRV particles to LamR, which was inferred from a VOPBA assay.

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2016

References

  • Analysis of green tea catechins: comparative study between HPLC and HPCE
    Bonoli, M; Pelillo, M; Toschi, TG; Lercker, G
  • Matrix metalloproteinase inhibition by green tea catechins
    Demeule, M; Brossard, M; Pagé, M; Gingras, D; Béliveau, R
  • Characterization of the nonstructural protein NS80 of grass carp reovirus
    Fan, C; Shao, L; Fang, Q

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