ISSN 1022-7954, Russian Journal of Genetics, 2006, Vol. 42, No. 6, pp. 662–666. © Pleiades Publishing, Inc., 2006.
Original Russian Text © L.G. Tyryshkin, E.I. Gul’tyaeva, N.V. Alpat’eva, I. Kramer, 2006, published in Genetika, 2006, Vol. 42, No. 6, pp. 812–817.
One of the most economical and environmentally
safe methods of controlling crop diseases involves the
use of varieties bearing effective resistance genes. Leaf
rust, caused by
Rob. ex. Desm.), is one of the major threats
L. in most areas of its pro-
duction. At present, 55 genes for leaf-rust resistance
) are known in the gene pool of
genes are highly effective in
Europe  and Russia . However, the wide cultivation
of varieties protected by
resulted in loss of its efﬁ-
ciency in the Volga and Volga–Vyatka regions [6, 7].
Previously, we identiﬁed 70 wheat accessions from
the worldwide collection of the Vavilov Institute of
Plant Industry (VIR) that were resistant to leaf rust at
the juvenile stage. Test crosses revealed the
genes in 25 of them .
ﬁed in 14 and 36 accessions, respectively, with test
clones. Test clones virulent against
leaf segments on water but not intact plants . In spite
of the strictly speciﬁc interaction between such clones
and wheat accessions reported to possess
identiﬁcation of this gene cannot be ruled out in this
gene was conjectured in plants not
affected by any pathogen clone or population [8–10],
although the presence of novel effective resistance
genes or combinations of known ones was conceivable.
Molecular DNA markers are a useful tool for selec-
tion of plants with certain genes in segregating popula-
tions. They are of special interest for pyramiding effec-
tive resistance genes, as lines bearing one or more such
genes are phenotypically indistinguishable after inocula-
tion with pathogens. Sequence-tagged-site (STS) mark-
ers J13, Gb, and J09, linked to the leaf-rust resistance
 were devel-
oped on the basis of RAPD, AFLP, and RFLP DNA
fragments. The high speciﬁcity of these markers was
shown in tests of a near-isogenic line (NIL) of cv.
Thatcher, bearing more than 40
Here we report application of STS markers for the
genes to testing wheat accessions from
the collection of the Vavilov Institute of Plant Industry
supposedly protected by these genes.
MATERIALS AND METHODS
Identiﬁcation of the
performed in 55 common wheat accessions from the
Vavilov Institute collection resistant to leaf rust at the
juvenile stage [8–10]. Susceptible cv. Thatcher was
used as a negative control, and its NILs with the
genes, as positive ones.
Intact plants at the stage of one or two leaves were
sprayed with water suspension of uredospores of a
complex population of the leaf-rust causal agent. The
population had been obtained by mixing samples from
damaged wheat plants in North Caucasus, the Volga
region, and northwestern Russia. The response to the
agent was scored on day 12 after inoculation according
to the Mains and Jackson scale: 0, absence of symp-
toms; 0, necrotic lesions without pustules; 1, very small
pustules bordered by necrosis; 2, medium-sized pus-
tules bordered by necrosis or chlorosis; 3, medium-
sized pustules without necrosis . Intact plants were
also tested for resistance to two
. The clones had been isolated by inoc-
ulation of leaf segments of an
-bearing Thatcher NIL
with the complex population of the leaf-rust agent [8, 10].
Their frequency was ca.
Leaf segments of accessions resistant to the popula-
-virulent clones were placed onto wet cot-
Identification of Effective Leaf-Rust Resistance Genes
in Wheat (
) Using STS Markers
L. G. Tyryshkin
, E. I. Gul’tyaeva
, N. V. Alpat’eva
, and I. Kramer
Vavilov Institute of Plant Industry, St. Petersburg, 190000 Russia;
All-Russia Institute of Plant Protection, St.-Petersburg–Pushkin, 196608 Russia
Institute of Epidemiology and Resistance Resources, Aschersleben, Germany
Received June 20, 2005
—Molecular STS markers J13, Gb, and J09 were used for screening wheat (
accessions previously found to possess leaf-rust resistance genes according to test crosses or phytopathological
tests. Speciﬁc ampliﬁcation products were detected in all accessions assumed to possess the
gene, in nine of
ten accessions with the conjectured
gene, and in 13 of 29 accessions with the conjectured
cation of STS markers to identiﬁcation of accessions possessing effective leaf-rust resistance genes is discussed.