Arch Virol (2003) 148: 2381–2396
Identiﬁcation of differentially expressed genes
in shrimp (Penaeus stylirostris) infected
with White spot syndrome virus by cDNA microarrays
A. K. Dhar
, A. Dettori
, M. M. Roux
, K. R. Klimpel
, and B. Read
Department of Biology, San Diego State University, San Diego,
Gen-Probe, San Diego, California, U.S.A.
Department of Molecular, Cellular and Developmental Biology,
University of California, Santa Barbara, California, U.S.A.
Aqua Bounty Paciﬁc Inc., Laboratory, San Diego, California, U.S.A.
Department of Biological Sciences, California State University,
San Marcos, California, U.S.A.
Received October 8, 2002; accepted June 11, 2003
Published online October 9, 2003
Summary. White spot syndrome virus (WSSV) is currently the most important vi-
ral pathogen infecting penaeid shrimp worldwide.Although considerable progress
has been made in characterizing the WSSV genome and developing detection
methods, information pertaining to host genes involved in WSSV pathogenesis is
limited. We examined the potential of cDNA microarray analysis to study gene
expression in WSSV-infected shrimp. Shrimp cDNAs were printed as low-density
arrays on glass slides and were hybridized with Cy3/Cy5 labeled probes derived
from RNA isolated from healthy and WSSV-infected shrimp. Genes that code for
proteins that are relevant to crustacean immunity, structural proteins, as well as
proteins of unknown function were among those whose mRNA expression was
altered upon WSSV infection. To validate the microarray data, the temporal ex-
pression of three differentially expressed genes, an immune gene (C-type lectin-1),
a structural gene (40S ribosomal protein), and a gene involved in lipid metabolism
(fatty acid binding protein) was measured in healthy and WSSV-infected shrimp
by real-time RT-PCR. The data suggest that WSSV infection alters the expression
of a wide array of cellular genes, and provides a framework for further studies
aimed at identifying genes whose function may provide insight into the mechanism
of WSSV infection in shrimp.