Identification of candidate genes underlying genic male-sterile msc-1 locus via genome resequencing in Capsicum annuum L.

Identification of candidate genes underlying genic male-sterile msc-1 locus via genome... Key message Based on genome resequencing, a strong candidate gene Capana02g002096 was identified in this study. Capana02g002096 encodes a homolog of AtDYT1 which is a bHLH transcription factor and involves in the early tapetal development. Abstract Genic male-sterile line is an efficient tool for commercial hybrid seed production in pepper; however, so far, only few genes controlling this trait have been cloned. A spontaneous genic male-sterile mutant, msc-1, had been identified and widely used in China, of which the male-sterile trait was proved to be controlled by a single recessive locus. For cloning the gene(s) underlying the msc-1 locus, genome resequencing and comparison analyses were performed between male-sterile and male-fertile lines. According to the genomic variations and genes’ annotations, Capana02g002096 was selected as a candidate gene underlying the msc-1 locus. Capana02g002096 encodes a homolog of AtDYT1, which is a bHLH transcription factor and involves in the early tapetal development. Moreover, a 7-bp deletion was identified in the exon of Capana02g002096, which led to a premature stop codon and may cause a loss-of-function mutation. Further genotyping in the 16C1369AB population containing 1110 plants, a F population consisting of 510 plants and 46 inbreed lines revealed that the male-sterile phenotype was http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

Identification of candidate genes underlying genic male-sterile msc-1 locus via genome resequencing in Capsicum annuum L.

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Life Sciences; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Agriculture; Plant Biochemistry; Biochemistry, general; Biotechnology
ISSN
0040-5752
eISSN
1432-2242
D.O.I.
10.1007/s00122-018-3119-1
Publisher site
See Article on Publisher Site

Abstract

Key message Based on genome resequencing, a strong candidate gene Capana02g002096 was identified in this study. Capana02g002096 encodes a homolog of AtDYT1 which is a bHLH transcription factor and involves in the early tapetal development. Abstract Genic male-sterile line is an efficient tool for commercial hybrid seed production in pepper; however, so far, only few genes controlling this trait have been cloned. A spontaneous genic male-sterile mutant, msc-1, had been identified and widely used in China, of which the male-sterile trait was proved to be controlled by a single recessive locus. For cloning the gene(s) underlying the msc-1 locus, genome resequencing and comparison analyses were performed between male-sterile and male-fertile lines. According to the genomic variations and genes’ annotations, Capana02g002096 was selected as a candidate gene underlying the msc-1 locus. Capana02g002096 encodes a homolog of AtDYT1, which is a bHLH transcription factor and involves in the early tapetal development. Moreover, a 7-bp deletion was identified in the exon of Capana02g002096, which led to a premature stop codon and may cause a loss-of-function mutation. Further genotyping in the 16C1369AB population containing 1110 plants, a F population consisting of 510 plants and 46 inbreed lines revealed that the male-sterile phenotype was

Journal

TAG Theoretical and Applied Genetics – Springer Journals

Published: May 31, 2018

References

  • Pollen exine pattern formation is dependent on three major developmental processes in Arabidopsis thaliana
    Ariizumi, T; Toriyama, K
  • Mapping of male sterility gene ms10 in chilli pepper (Capsicum annuum L.)
    Aulakh, PS; Dhaliwal, MS; Jindal, SK; Schafeitner, R; Singh, K
  • Mapping of the ms8 male sterility gene in sweet pepper (Capsicum annuum L.) on the chromosome P4 using PCR-based markers useful for breeding programmes
    Bartoszewski, G; Waszczak, C; Gawroński, P; Stępień, I; BolibokBrągoszewska, H; Palloix, A; Lefebvre, V; Korzeniewska, A; Niemirowicz-Szczytt, K
  • Male sterility and fertility restoration in crops
    Chen, L; Liu, Y
  • A method of high frequency virus-induced gene silencing in chili pepper (Capsicum annuum L. cv. Bukang)
    Chung, E; Seong, E; Kim, YC; Chung, EJ; Oh, SK; Lee, S; Park, JM; Joung, YH; Choi, D
  • Induction and exploitation of nuclear and cytoplasmic male sterility in pepper (Capsicum spp.): a review
    Dhaliwal, MS; Jindal, SK
  • Common mode of deposition for the sporopollenin of sexine and nexine
    Dickinson, HG; Heslop-Harrison, J
  • Genetic and molecular characterization of photoperiod and thermo-sensitive male sterility in rice
    Fan, Y; Zhang, Q
  • A single point mutation in Ms44 results in dominant male sterility and improves nitrogen use efficiency in maize
    Fox, T; DeBruin, J; Haug Collet, K; Trimnell, M; Clapp, J; Leonard, A; Li, B; Scolaro, E; Collinson, S
  • DYT1 directly regulates the expression of TDF1 for tapetum development and pollen wall formation in Arabidopsis
    Gu, JN; Zhu, J; Yu, Y; Teng, XD; Lou, Y; Xu, XF; Liu, JL; Yang, ZN
  • Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant
    Han, Y; Zhao, F; Gao, S; Wang, X; Wei, A; Chen, Z; Liu, N; Tong, X; Fu, X; Wen, C; Zhang, Z; Wang, N; Du, S
  • Fine mapping of a recessive genic male sterility gene (Bnms3) in rapeseed (Brassica napus) with AFLP- and Arabidopsis-derived PCR markers
    He, J; Ke, L; Hong, D; Xie, Y; Wang, G; Liu, P; Yang, G
  • Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
    Healey, A; Furtado, A; Cooper, T; Henry, RJ
  • Origin of exine
    Heslop-Harrison, J
  • MMAPPR: mutation mapping analysis pipeline for pooled RNA-seq
    Hill, JT; Demarest, BL; Bisgrove, BW; Gorsi, B; Su, YC; Yost, HJ
  • Fine mapping of the recessive genic male sterility gene (Bnms3) in Brassica napus L
    Huang, Z; Chen, Y; Yi, B; Xiao, L; Ma, C; Tu, J; Fu, T
  • Arabidopsis MALE STERILITY1 encodes a PHD-type transcription factor and regulates pollen and tapetum development
    Ito, T; Nagata, N; Yoshiba, Y; Ohme-Takagi, M; Ma, H; Shinozaki, K
  • Tomato Male-sterile 10 35 is essential for pollen development and meiosis in anthers
    Jeong, HJ; Kang, JH; Zhao, M; Kwon, JK; Choi, HS; Bae, JH; Lee, HA; Joung, YH; Choi, D; Kang, BC
  • Fine mapping of the genic male-sterile ms 1 gene in Capsicum annuum L
    Jeong, K; Choi, D; Lee, J
  • Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice
    Jia, JH; Zhang, DS; Li, CY; Qu, XP; Wang, SW; Chamarerk, V; Nguyen, HT; Wang, B
  • Comparative embryology of angiosperms
    Johri, BM; Ambegaokar, KB; Srivastava, PS
  • Rice undeveloped Tapetum1 is a major regulator of early tapetum development
    Jung, KH; Han, MJ; Lee, YS; Kim, YW; Hwang, I; Kim, MJ; Kim, YK; Nahm, BH; An, G
  • Molecular control of male fertility for crop hybrid breeding
    Kim, YJ; Zhang, D
  • Genetics and distribution of fertility restoration associated RAPD markers in pepper (Capsicum annuum L.)
    Kumar, S; Singh, V; Singh, M; Rai, S; Kumar, S; Rai, SK; Rai, M
  • MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets
    Kumar, S; Stecher, G; Tamura, K
  • A CAPS marker linked to a genic male-sterile gene in the colored sweet pepper, ‘Paprika’ (Capsicum annuum L.)
    Lee, J; Han, JH; An, CG; Lee, WP; Yoon, JB
  • Development of molecular marker linked to a genic male-sterile gene, ms k in chili pepper
    Lee, J; Lee, WP; Han, JH; Yoon, JB
  • Three AFLP markers tightly linked to the genic male sterility ms 3 gene in chili pepper (Capsicum annuum L.) and conversion to a CAPS marker
    Lee, J; Yoon, JB; Han, JH; Lee, WP; Kim, SH; Park, HG
  • Allelism and molecular marker tests for genic male sterility in paprika cultivars
    Lee, J; Do, JW; Han, JH; An, CG; Kweon, OY; Kim, YK; Yoon, JB
  • Development of a high resolution melting (HRM) marker linked to genic male sterility in Capsicum annuum L
    Lee, HR; An, HJ; Yang, DC; Choi, SH; Kim, HJ
  • Towards map based cloning: fine mapping of a recessive genic male-sterile gene (BnMs2) in Brassica napus L. and syntenic region identification based on the Arabidopsis thaliana genome sequences
    Lei, S; Yao, X; Yi, B; Chen, W; Ma, C; Tu, J; Fu, T
  • Fast and accurate short read alignment with Burrows-Wheeler transform
    Li, H; Durbin, R
  • The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development
    Li, N; Zhang, DS; Liu, HS; Yin, CS; Li, XX; Liang, WQ; Yuan, Z; Xu, B; Chu, HW; Wang, J; Wen, TQ; Huang, H; Luo, D; Ma, H; Zhang, DB
  • Map-based cloning of a recessive genic male sterility locus in Brassica napus L. and development of its functional marker
    Li, J; Hong, D; He, J; Ma, L; Wan, L; Liu, P; Yang, G
  • Map-based cloning of the dominant genic male sterile Ms-cd1 gene in cabbage (Brassica oleracea)
    Liang, J; Ma, Y; Wu, J; Cheng, F; Liu, B; Wang, X
  • Transcriptome sequencing and de novo analysis of a cytoplasmic male-sterile line and its near-isogenic restorer line in chili pepper (Capsicum annuum L.)
    Liu, C; Ma, N; Wang, PY; Fu, N; Shen, HL
  • Induction of male sterility in plants by achimeric ribonuclease gene
    Mariani, C; Beuckeleer, M; Truettner, J; Leemans, J; Goldberg, RB
  • The genome analysis toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data
    McKenna, A; Hanna, M; Banks, E; Sivachenko, A; Cibulskis, K; Kernytsky, A; Garimella, K; Altshuler, D; Gabriel, S; Daly, M; DePristo, MA
  • Molecular mapping of two reverse photoperiodsensitive genic male sterility genes (rpms1 and rpms2) in rice (Oryza sativa L.)
    Peng, HF; Zhang, ZF; Wu, B; Chen, XH; Zhang, GQ; Zhang, ZM; Wan, BH; Lu, YP
  • Fine mapping of a gene for non-pollen type thermosensitive genic male sterility in rice (Oryza sativa L.)
    Peng, HF; Chen, XH; Lu, YP; Peng, YF; Wan, BH; Chen, ND; Wu, B; Xin, SP; Zhang, GQ
  • Hybrid seed production and the challenge of propagating male-sterile plants
    Perez-Prat, E; Lookeren Campagne, MM
  • Biogenesis and function of the lipidic structures of pollen grains
    Piffanelli, P; Ross, JHE; Murphy, DJ
  • Phenotypic, genetic and molecular characterization of 7B–1, a conditional male-sterile mutant in tomato
    Pucci, A; Picarella, ME; Mazzucato, A
  • Fine mapping and candidate gene analysis of the novel thermo-sensitive genic male sterility tms9-1 gene in rice
    Qi, Y; Liu, Q; Zhang, L; Mao, B; Yan, D; Jin, Q; He, Z
  • Whole-genome sequencing of cultivated and wild peppers provides insights into Capsicum domestication and specialization
    Qin, C; Yu, C; Shen, Y; Fang, X; Chen, L; Min, J; Cheng, J; Zhao, S; Xu, M
  • Molecular embryology of flowering plants
    Raghavan, V
  • Male sterility in pepper (Capsicum annuum L.)
    Shifriss, C
  • CMS system and its stimulation in hybrid seed production of Capsicum annuum L
    Swamy, BN; Hedau, NK; Chaudhari, GV; Kant, L; Pattanayak, A
  • The genes of Capsicum
    Wang, D; Bosland, PW
  • Fine mapping of the rice thermo-sensitive genic male-sterile gene tms5
    Wang, YG; Xing, QH; Deng, QY; Liang, FS; Yuan, LP; Weng, ML; Wang, B
  • Qtl analysis of fertility restoration in cytoplasmic male-sterile pepper
    Wang, LH; Zhang, BX; Lefebvre, V; Huang, SW; Daubeze, AM; Palloix, A
  • Maize Male sterile 8 (Ms8), a putative β-1,3-galactosyltransferase, modulates cell division, expansion, and differentiation during early maize anther development
    Wang, DX; Skibbe, DS; Walbot, V
  • Map-based cloning and characterization of Zea mays male sterility33 (ZmMs33) gene, encoding a glycerol-3-phosphate acyltransferase
    Xie, K; Wu, S; Li, Z; Zhou, Y; Zhang, D; Dong, Z; An, X; Zhu, T; Zhang, S; Liu, S; Li, J; Wan, X
  • The ABORTED MICROSPORES regulatory network is required for postmeiotic male reproductive development in Arabidopsis thaliana
    Xu, J; Yang, C; Yuan, Z; Zhang, D; Gondwe, MY; Ding, Z; Liang, W; Zhang, D; Wilson, ZA
  • Fine mapping and candidate gene analysis of ptgms2-1, the photoperiod-thermo-sensitive genic male sterile gene in rice (Oryza sativa L.)
    Xu, J; Wang, B; Wu, Y; Du, P; Wang, J; Wang, M; Yi, C; Gu, M; Liang, G
  • ABORTED MICROSPORES acts as a master regulator of pollen wall formation in Arabidopsis
    Xu, J; Ding, Z; Vizcay-Barrena, G; Shi, J; Liang, W; Yuan, Z; Werck-Reichhart, D; Schreiber, L; Wilson, ZA; Zhang, D
  • Fine mapping of the recessive genic male-sterile gene (Bnms1) in Brassica napus L
    Yi, B; Chen, Y; Lei, S; Tu, J; Fu, T
  • Two duplicate CYP704B1-homologous genes BnMs1 and BnMs2 are required for pollen exine formation and tapetal development in Brassica napus
    Yi, B; Zeng, F; Lei, S; Chen, Y; Yao, X; Zhu, Y; Wen, J; Shen, J; Ma, C; Tu, J; Fu, T
  • Fine mapping of a dominant thermo-sensitive genic male sterility gene (BntsMs) in rapeseed (Brassica napus) with AFLP- and Brassica rapa-derived PCR markers
    Zeng, X; Li, W; Wu, Y; Liu, F; Luo, J; Cao, Y; Zhu, L; Li, Y; Li, J; You, Q; Wu, G
  • Two RAPD markers linked to a major fertility restorer gene in pepper
    Zhang, BX; Huang, SW; Yang, GM; Guo, JZ
  • Regulation of Arabidopsis tapetum development and function by DYSFUNCTIONAL TAPETUM1 (DYT1) encoding a putative bHLH transcription factor
    Zhang, W; Sun, Y; Timofejeva, L; Chen, C; Grossniklaus, U; Ma, H
  • Transcription factor AtMYB103 is required for anther development by regulating tapetum development, callose dissolution and exine formation in Arabidopsis
    Zhang, ZB; Zhu, J; Gao, JF; Wang, C; Li, H; Li, H; Zhang, HQ; Zhang, S; Wang, DM; Wang, QX; Huang, H; Xia, HJ; Yang, ZN
  • Construction of a multi-control sterility system for a maize male-sterile line and hybrid seed production based on the ZmMs7 gene encoding a PHD-finger transcription factor
    Zhang, D; Wu, S; An, X; Xie, K; Dong, Z; Zhou, Y; Xu, L; Fang, W; Liu, S; Liu, S; Zhu, T; Li, J; Rao, L; Zhao, J; Wan, X
  • Photoperiod- and thermosensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA
    Zhou, H; Liu, Q; Li, J; Jiang, D; Zhou, L; Wu, P; Lu, S; Li, F; Zhu, L; Liu, Z; Chen, L; Liu, YG; Zhuang, C
  • RNase ZS1 processes Ub L40 mRNAs and controls thermosensitive genic male sterility in rice
    Zhou, H; Zhou, M; Yang, Y; Li, J; Zhu, L; Jiang, D; Dong, J; Liu, Q; Gu, L
  • Defective in Tapetal development and function 1 is essential for anther development and tapetal function for microspore maturation in Arabidopsis
    Zhu, J; Chen, H; Li, H; Gao, JF; Jiang, H; Wang, C; Guan, YF; Yang, ZN
  • A genetic pathway for tapetum development and function in Arabidopsis
    Zhu, J; Lou, Y; Xu, X; Yang, ZN

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