Plant Mol Biol (2017) 94:167–183 DOI 10.1007/s11103-017-0600-1 Identification of alternatively spliced transcripts of rice phytochelatin synthase 2 gene OsPCS2 involved in mitigation of cadmium and arsenic stresses 1 1,3 2 Natasha Das · Surajit Bhattacharya · Somnath Bhattacharyya · Mrinal K. Maiti Received: 10 December 2016 / Accepted: 28 February 2017 / Published online: 11 March 2017 © Springer Science+Business Media Dordrecht 2017 Abstract yeast cells. An intron-containing hairpin RNA-mediated Key message The OsPCS2 exhibits root- and shoot- gene silencing was carried out in endosperm-specific man- specific differential ratios of alternatively spliced tran- ner for efficient down-regulation of OsPCS genes in rice scripts in indica rice under Cd stress, and plays role in grains. Analysis of the transgenic rice lines grown under Cd and As stress tolerance and accumulation. metal(loid) stress revealed almost complete absence of Abstract Enzymatic activity of phytochelatin synthase both OsPCS1 and OsPCS2 transcripts in the developing (PCS) in plant produces phytochelatins, which help in seeds coupled with the significant reduction in the content sequestration of heavy metal(loid)s inside the cell vacuole of Cd (~51%) and As (~35%) in grains compared with the to alleviate toxicity. Here we report that among the two non-transgenic plant. Taken together, the findings indicate PCS genes—OsPCS1 and OsPCS2 in
Plant Molecular Biology – Springer Journals
Published: Mar 11, 2017
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