Viral replication and late gene expression of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) requires a series of late expression factors. We examined the AcMNPV genome for additional factors which affect late gene expression. During these investigations, a segment of the AcMNPV genome located immediately downstream to LEF-7 was found to possess a repressor activity. Elimination of this region resulted in greatly enhanced late gene expression. We were unable to link repressor activity to a single open reading frame, suggesting that a cis -acting element rather than a trans -acting factor is responsible for this phenomenon.
Archives of Virology – Springer Journals
Published: Jun 1, 2003
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