Identification of a novel B-cell epitope specific for avian leukosis virus subgroup J gp85 protein

Identification of a novel B-cell epitope specific for avian leukosis virus subgroup J gp85 protein Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that has caused severe economic losses in China. Gp85 protein is the main envelope protein and the most variable structural protein of ALV-J. It is also involved in virus neutralization. In this study, a specific monoclonal antibody, 4A3, was produced against the ALV-J gp85 protein. Immunofluorescence assays showed that 4A3 could react with different strains of ALV-J, including the British prototype isolate HPRS103, the American strains, an early Chinese broiler isolate, and layer isolates. A linear epitope on the gp85 protein was identified using a series of partially overlapping fragments spanning the gp85-encoding gene and subjecting them to western blot analysis. The results indicated that 134 AEAELRDFI 142 was the minimal linear epitope that could be recognized by mAb 4A3. Enzyme-linked immunosorbent assay (ELISA) revealed that chicken anti-ALV-J sera and mouse anti-ALV-J gp85 sera could also recognize the minimal linear epitope. Alignment analysis of amino acid sequences indicated that the epitope was highly conserved among 34 ALV-J strains. Furthermore, the epitope was not conserved among subgroup A and B of avian leukosis virus (ALV). Taken together, the mAb and the identified epitope may provide valuable tools for the development of new diagnostic methods for ALV-J. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Identification of a novel B-cell epitope specific for avian leukosis virus subgroup J gp85 protein

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2318-6
Publisher site
See Article on Publisher Site

Abstract

Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that has caused severe economic losses in China. Gp85 protein is the main envelope protein and the most variable structural protein of ALV-J. It is also involved in virus neutralization. In this study, a specific monoclonal antibody, 4A3, was produced against the ALV-J gp85 protein. Immunofluorescence assays showed that 4A3 could react with different strains of ALV-J, including the British prototype isolate HPRS103, the American strains, an early Chinese broiler isolate, and layer isolates. A linear epitope on the gp85 protein was identified using a series of partially overlapping fragments spanning the gp85-encoding gene and subjecting them to western blot analysis. The results indicated that 134 AEAELRDFI 142 was the minimal linear epitope that could be recognized by mAb 4A3. Enzyme-linked immunosorbent assay (ELISA) revealed that chicken anti-ALV-J sera and mouse anti-ALV-J gp85 sera could also recognize the minimal linear epitope. Alignment analysis of amino acid sequences indicated that the epitope was highly conserved among 34 ALV-J strains. Furthermore, the epitope was not conserved among subgroup A and B of avian leukosis virus (ALV). Taken together, the mAb and the identified epitope may provide valuable tools for the development of new diagnostic methods for ALV-J.

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2015

References

  • Isolation and characterization of emerging subgroup J avian leukosis virus associated with hemangioma in egg-type chickens
    Lai, H; Zhang, H; Ning, Z; Chen, R; Zhang, W; Qing, A; Yu, K; Cao, W; Liao, M
  • Genetic diversity and phylogenetic analysis of glycoprotein GP85 of ALV-J isolates from Mainland China between 1999 and 2010: coexistence of two different subgroups in layers
    Pan, W; Gao, YL; Qin, LT; Ni, W; Liu, ZS; Yun, BL; Wang, YQ; Qi, XL; Gao, HL; Wang, XM
  • Identification of B cell epitopes of dengue virus 2 NS3 protein by monoclonal antibody
    Tian, Y; Chen, W; Yang, Y; Xu, X; Zhang, J; Wang, J; Xiao, L; Chen, Z
  • Specificity, polyspecificity, and heterospecificity of antibody-antigen recognition
    Regenmortel, MHV
  • Use of reverse transcriptase polymerase chain reaction for detection of vaccine contamination by avian leukosis virus
    Hauptli, D; Bruckner, L; Ottiger, HP
  • Development and application of polymerase chain reaction (PCR) tests for the detection of subgroup J avian leukosis virus
    Smith, LM; Brown, SR; Howes, K; McLeod, S; Arshad, SS; Barron, GS; Venugopal, K; McKay, JC; Payne, LN

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