Identification of a new Apscaviroid from Japanese persimmon

Identification of a new Apscaviroid from Japanese persimmon Three viroid-like sequences were detected from Japanese persimmon ( Diospyrus kaki Thunb.) by RT-PCR using primers specific for members of the genus Apscaviroid . Based on the sequences, we determined the complete genomic sequences. Two had 92.1–94.3% sequence identity with citrus viroid OS (CVd-OS) and 91.4–96.3% identity with apple fruit crinkle viroid (AFCVd), respectively. Another one, tentatively named persimmon viroid (PVd), had 396 nucleotides and less than 70% sequence identity with known viroids. The secondary structure of PVd is proposed to be rod-like with extensive base pairing and contains the terminal conserved region and the central conserved region characteristic of the genus Apscaviroid . Moreover, we confirmed that the viroids, including PVd, are graft transmissible from persimmon to persimmon and that persimmon is a natural host of these viroids. According to its molecular and biological properties, PVd should be considered a member of a new species in the genus Apscaviroid . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Identification of a new Apscaviroid from Japanese persimmon

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Publisher
Springer Journals
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-008-0073-2
Publisher site
See Article on Publisher Site

Abstract

Three viroid-like sequences were detected from Japanese persimmon ( Diospyrus kaki Thunb.) by RT-PCR using primers specific for members of the genus Apscaviroid . Based on the sequences, we determined the complete genomic sequences. Two had 92.1–94.3% sequence identity with citrus viroid OS (CVd-OS) and 91.4–96.3% identity with apple fruit crinkle viroid (AFCVd), respectively. Another one, tentatively named persimmon viroid (PVd), had 396 nucleotides and less than 70% sequence identity with known viroids. The secondary structure of PVd is proposed to be rod-like with extensive base pairing and contains the terminal conserved region and the central conserved region characteristic of the genus Apscaviroid . Moreover, we confirmed that the viroids, including PVd, are graft transmissible from persimmon to persimmon and that persimmon is a natural host of these viroids. According to its molecular and biological properties, PVd should be considered a member of a new species in the genus Apscaviroid .

Journal

Archives of VirologySpringer Journals

Published: May 1, 2008

References

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