Three viroid-like sequences were detected from Japanese persimmon ( Diospyrus kaki Thunb.) by RT-PCR using primers specific for members of the genus Apscaviroid . Based on the sequences, we determined the complete genomic sequences. Two had 92.1–94.3% sequence identity with citrus viroid OS (CVd-OS) and 91.4–96.3% identity with apple fruit crinkle viroid (AFCVd), respectively. Another one, tentatively named persimmon viroid (PVd), had 396 nucleotides and less than 70% sequence identity with known viroids. The secondary structure of PVd is proposed to be rod-like with extensive base pairing and contains the terminal conserved region and the central conserved region characteristic of the genus Apscaviroid . Moreover, we confirmed that the viroids, including PVd, are graft transmissible from persimmon to persimmon and that persimmon is a natural host of these viroids. According to its molecular and biological properties, PVd should be considered a member of a new species in the genus Apscaviroid .
Archives of Virology – Springer Journals
Published: May 1, 2008
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