Identification of a Mutant Gene Controlling Necrotic Cotyledons in Developing Seedlings of Arabidopsis thaliana

Identification of a Mutant Gene Controlling Necrotic Cotyledons in Developing Seedlings of... Genetic and molecular analyses of an Arabidopsis thalianamutant with necrotic cotyledons from the collection of insertion mutants obtained earlier were conducted. The mutation under study showed incomplete dominance and represented a single insertion of the T region of pLD3 vector used for transformation of germinating seeds to the plant genome during the creation of the collection. Using TAIL–PCR, a fragment of the mutant DNA adjacent to the left border of the T-DNA insertion was isolated and sequenced. Computer r-aided analysis showed that the insertion was located on the left arm of chromosome 1. The open reading frame containing the insertion has one exon and encodes a protein of 446 amino acids, whose function is unknown. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Identification of a Mutant Gene Controlling Necrotic Cotyledons in Developing Seedlings of Arabidopsis thaliana

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1016650309282
Publisher site
See Article on Publisher Site

Abstract

Genetic and molecular analyses of an Arabidopsis thalianamutant with necrotic cotyledons from the collection of insertion mutants obtained earlier were conducted. The mutation under study showed incomplete dominance and represented a single insertion of the T region of pLD3 vector used for transformation of germinating seeds to the plant genome during the creation of the collection. Using TAIL–PCR, a fragment of the mutant DNA adjacent to the left border of the T-DNA insertion was isolated and sequenced. Computer r-aided analysis showed that the insertion was located on the left arm of chromosome 1. The open reading frame containing the insertion has one exon and encodes a protein of 446 amino acids, whose function is unknown.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 16, 2004

References

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