Identification and molecular characterization of Panax notoginseng virus A, which may represent an undescribed novel species of the genus Totivirus , family Totiviridae

Identification and molecular characterization of Panax notoginseng virus A, which may represent... A double-stranded RNA (dsRNA), PN-S, isolated from Panax notoginseng was sequenced and characterized. dsRNA PN-S was determined to be 5003 base pairs (bp) in length and to have a genome organization similar to that of totiviruses. The two deduced proteins encoded by dsRNA PN-S have the highest amino acid (aa) sequence identity to CP and RNA-dependent RNA polymerase (RdRp) of black raspberry virus F (BrVF), a tentative member of the genus Totivirus, family Totiviridae , at 39 % and 54 %, respectively. In a phylogenetic tree based on an alignment of the RdRp amino acid sequences of dsRNA PN-S and selected totiviruses, dsRNA PN-S formed a monophyletic clade with dsRNA viruses belonging to the genus Totivirus. These data suggest that dsRNA PN-S may represent the genome of a member of a novel species of the genus Totivirus , family Totiviridae , for which we propose the name Panax notoginseng virus A (PnVA) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Identification and molecular characterization of Panax notoginseng virus A, which may represent an undescribed novel species of the genus Totivirus , family Totiviridae

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2716-4
Publisher site
See Article on Publisher Site

Abstract

A double-stranded RNA (dsRNA), PN-S, isolated from Panax notoginseng was sequenced and characterized. dsRNA PN-S was determined to be 5003 base pairs (bp) in length and to have a genome organization similar to that of totiviruses. The two deduced proteins encoded by dsRNA PN-S have the highest amino acid (aa) sequence identity to CP and RNA-dependent RNA polymerase (RdRp) of black raspberry virus F (BrVF), a tentative member of the genus Totivirus, family Totiviridae , at 39 % and 54 %, respectively. In a phylogenetic tree based on an alignment of the RdRp amino acid sequences of dsRNA PN-S and selected totiviruses, dsRNA PN-S formed a monophyletic clade with dsRNA viruses belonging to the genus Totivirus. These data suggest that dsRNA PN-S may represent the genome of a member of a novel species of the genus Totivirus , family Totiviridae , for which we propose the name Panax notoginseng virus A (PnVA)

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2016

References

  • Sequence and genome organization of papaya meleira virus infecting papaya in Brazil
    Abreu, EFM; Daltro, CB; Nogueira, EOPL; Andrade, EC; Aragão, FJL
  • Tracking the changes in virus taxonomy
    Adams, MJ; Hendrickson, RC; Dempsey, DM; Lefkowitz, EJ
  • Victorivirus, a new genus of fungal viruses in the family Totiviridae
    Ghabrial, SA; Nibert, ML

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