Identification and characterization of new microRNAs from pig

Identification and characterization of new microRNAs from pig MicroRNAs (miRNAs) are small regulatory RNAs that direct the posttranscriptional repression of cognate messenger RNAs. Despite increasing evidence for diverse roles of miRNAs in biological processes, little is known about miRNAs in pig. We describe the first experimental identification of porcine miRNAs by sequence analysis of a cDNA library of small RNAs from porcine fibroblast cells. We identified 25 distinct porcine miRNAs, of which 19 are previously unreported, and define 14 new miRNA families in pig. Most of the cloned miRNAs are expressed ubiquitously in all porcine tissues examined, whereas some miRNAs are expressed preferentially in specific tissues. Our results enrich the porcine miRNA database and provide useful information for investigating biological functions of miRNAs in pig. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Identification and characterization of new microRNAs from pig

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Publisher
Springer-Verlag
Copyright
Copyright © 2008 by Springer Science+Business Media, LLC
Subject
Life Sciences; Zoology ; Anatomy ; Cell Biology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s00335-008-9111-3
Publisher site
See Article on Publisher Site

Abstract

MicroRNAs (miRNAs) are small regulatory RNAs that direct the posttranscriptional repression of cognate messenger RNAs. Despite increasing evidence for diverse roles of miRNAs in biological processes, little is known about miRNAs in pig. We describe the first experimental identification of porcine miRNAs by sequence analysis of a cDNA library of small RNAs from porcine fibroblast cells. We identified 25 distinct porcine miRNAs, of which 19 are previously unreported, and define 14 new miRNA families in pig. Most of the cloned miRNAs are expressed ubiquitously in all porcine tissues examined, whereas some miRNAs are expressed preferentially in specific tissues. Our results enrich the porcine miRNA database and provide useful information for investigating biological functions of miRNAs in pig.

Journal

Mammalian GenomeSpringer Journals

Published: Jun 12, 2008

References

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