Identification and characterization of mutations in the high growth vaccine strain of influenza virus

Identification and characterization of mutations in the high growth vaccine strain of influenza... X-31(H3N2) virus, which is a high yielding reassortant between A/PR/8/34(H1N1) and A/Aichi/68(H3N2), is currently used as a backbone strain for influenza vaccine production. The sequence of the current X-31 virus was determined from cloned cDNA of 6 internal RNA genes, and was compared with the original sequence of the A/PR/8/34 virus. 71 point mutations were accumulated in the six internal viral genes (PB2, PB1, PA, NP, M and NS). These nucleotide changes encode 23 amino acid substitutions in seven viral proteins (PB2, PB1, PA, M1, M2, NS1 and NS2). Among three polymerase genes, a significantly low mutation frequency was observed in PA gene as compared to PB2 and PB1. The mutation frequency at the nucleotide level was significantly low in NP gene without any amino acid substitution, being only about 20% of those observed in 5 other internal genes. The unequal distribution of mutations among different viral proteins may correlate with individual role of each protein in viral growth. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Identification and characterization of mutations in the high growth vaccine strain of influenza virus

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Publisher
Springer-Verlag
Copyright
Copyright © 2001 by Springer-Verlag/Wien
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050170181
Publisher site
See Article on Publisher Site

Abstract

X-31(H3N2) virus, which is a high yielding reassortant between A/PR/8/34(H1N1) and A/Aichi/68(H3N2), is currently used as a backbone strain for influenza vaccine production. The sequence of the current X-31 virus was determined from cloned cDNA of 6 internal RNA genes, and was compared with the original sequence of the A/PR/8/34 virus. 71 point mutations were accumulated in the six internal viral genes (PB2, PB1, PA, NP, M and NS). These nucleotide changes encode 23 amino acid substitutions in seven viral proteins (PB2, PB1, PA, M1, M2, NS1 and NS2). Among three polymerase genes, a significantly low mutation frequency was observed in PA gene as compared to PB2 and PB1. The mutation frequency at the nucleotide level was significantly low in NP gene without any amino acid substitution, being only about 20% of those observed in 5 other internal genes. The unequal distribution of mutations among different viral proteins may correlate with individual role of each protein in viral growth.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2001

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