Nucleotide and amino acid variability of fragments of the Sus4 gene encoding the sucrose synthase enzyme was studied in 24 potato cultivars bred in Russia and other countries and differing in starch content in tubers. Both SNPs and indels were detected in a chosen Sus4 gene fragment including the sequence from exon 3 to exon 6 and corresponding to the main part of the sucrose synthase domain. Four types of Sus4 sequences were revealed depending on the presence of an insertion in introns 4 and 5 and of the mononucleotide octamer (T)8 in intron 5. Differentiation of these sequences was confirmed by statistical methods. Sixteen amino acid substitutions were identified in the translated sequence, of which eleven were nonsynonymous. Specific cultivar-specific nucleotide and amino acid substitutions were also revealed, which can be used in future for identifying potato cultivars/genotypes. No direct associations between the mutational changes and the starch content were found in the potato cultivars studied.
Russian Journal of Genetics – Springer Journals
Published: Feb 22, 2011
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