Identification and characterization of a new virus in the genus Potyvirus from wild populations of Angelica lucida L. and A. genuflexa Nutt., family Apiaceae

Identification and characterization of a new virus in the genus Potyvirus from wild populations... A novel potyvirus was discovered in Angelica lucida L. (wild celery) and A. genuflexa Nutt. (kneeling Angelica) (family Apiaceae ) in the Matanuska- Susitna Valley, Alaska. The experimental plant host range of the virus included species in three families: Chenopodiaceae ( Chenopodium amaranticolor Coste et Reyn and Chenopodium quinoa Willd.), Solanaceae ( Nicotiana benthamiana Domin, and N. clevlandii Gray.), and Apiaceae ( Anethum graveolens L., Apium graveolens L. var. dulce (Miller)), Daucus carota L. subspecies sativus (Hoffm.) Arcang., and Petroselinum crispum (Miller) Nyman ex A. W. Hilland). The virus contained flexuous rods with an ssRNA genome ∼9.5–10 k nts and a CP (∼35 kDa) that reacted to a universal potyvirus monoclonal antibody in Western blot analysis. The sequenced genomic 3′-end (∼1850 nt) contained a potyvirus genomic arrangement that included the 3′-terminus of the NIb (nuclear inclusion) gene, the CP (coat protein) gene, and a 3′-UTR (untranslated region) attached to a poly(A)tail. The CP amino acids had between 54 and 70% identity with 12 selected members from the genus Potyvirus . Phylogenetically, the Alaskan potyvirus clustered with three other apiaceous potyviruses from Australia. The novel Alaskan virus was confined to A. lucida L. and A. genuflexa Nutt. in nature, and was classified in the genus Potyvirus , family Potyviridae , and in part named after its natural plant hosts, angelica virus Y (AnVY). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Identification and characterization of a new virus in the genus Potyvirus from wild populations of Angelica lucida L. and A. genuflexa Nutt., family Apiaceae

Loading next page...
 
/lp/springer_journal/identification-and-characterization-of-a-new-virus-in-the-genus-NG530E48GN
Publisher
Springer Journals
Copyright
Copyright © 2007 by Springer-Verlag
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-007-1003-4
Publisher site
See Article on Publisher Site

Abstract

A novel potyvirus was discovered in Angelica lucida L. (wild celery) and A. genuflexa Nutt. (kneeling Angelica) (family Apiaceae ) in the Matanuska- Susitna Valley, Alaska. The experimental plant host range of the virus included species in three families: Chenopodiaceae ( Chenopodium amaranticolor Coste et Reyn and Chenopodium quinoa Willd.), Solanaceae ( Nicotiana benthamiana Domin, and N. clevlandii Gray.), and Apiaceae ( Anethum graveolens L., Apium graveolens L. var. dulce (Miller)), Daucus carota L. subspecies sativus (Hoffm.) Arcang., and Petroselinum crispum (Miller) Nyman ex A. W. Hilland). The virus contained flexuous rods with an ssRNA genome ∼9.5–10 k nts and a CP (∼35 kDa) that reacted to a universal potyvirus monoclonal antibody in Western blot analysis. The sequenced genomic 3′-end (∼1850 nt) contained a potyvirus genomic arrangement that included the 3′-terminus of the NIb (nuclear inclusion) gene, the CP (coat protein) gene, and a 3′-UTR (untranslated region) attached to a poly(A)tail. The CP amino acids had between 54 and 70% identity with 12 selected members from the genus Potyvirus . Phylogenetically, the Alaskan potyvirus clustered with three other apiaceous potyviruses from Australia. The novel Alaskan virus was confined to A. lucida L. and A. genuflexa Nutt. in nature, and was classified in the genus Potyvirus , family Potyviridae , and in part named after its natural plant hosts, angelica virus Y (AnVY).

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 2007

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off