Identification and characterization of a microsatellite marker within murine Brca2 gene

Identification and characterization of a microsatellite marker within murine Brca2 gene Mammalian Genome 8, Brief Data Reports 79 5. Kitamura, N., Kitagawa, H., Fukushima, D., Takagaki, Y., Miyata, T., Nakanishi, S., (1995). J. Biol. Chem. 260, 8610--8617. 6. Kitamura, N., Takagaki, Y., Furuto, S., Tanaka, T., Nawa, H., Nakani- shi, S. (1983). Nature 305, 545-549. 7. Harlizius, B., Hetzel, J., Barendse, W. (1995). Mamm. Genome 6, 486--483. 8. Moody, D.E., Pomp, D., Barendse, W. (1995). Anim. Genet. 26, 45- 9. Solinas-Toldo, S., Lengauer, C., Fries, R. (1995). Genomics 27, 489- 10. Threadgill, D.S., Womack. J.E. (1991). Genomics 11, 1143-1148. 11. Irie, Y., Tatsumi, K., Notomi, T., Endo, Y., Onogi, S., Miyai, K., Amino, N. (1993). Human Genome Mapping Workshop 93, 57. 12. Qumsieh, M.B., Valentine, M.B., Suttle, D.P. (1992). Genomics 5, Fig. 2. Polymorphism detection with PCR. Shown here are the PCR prod- 160-162. ucts amplified from the genomic DNA of Mus musculus, M. spretus, and 13. van Rens, G.L.M., Raats, J.M.H., Driessen, H.P.C., Oldenburg, M., M. musculus/M, spretus hybrid mice and separated on a 4% agarose gel in Wijnen, J.T., Meera Khan, P., de Jong, W.W., Bloemendal, H. (1989). lx TBE buffer. Gene 78, 225-233. 14. Cox, D.R., Kawashima, H., Vora, S., Epstein, C.J. (1984). Cytogenet. was found to be polymorphic between Mus musculus and Mus Cell Genet. 37, 441--442. spretus species (Fig. 2). Such a polymorphic marker within in a 15. Hawkins, J.W., Van Keuren, M.L., Piatigorshy, J., Law, M.L., Patter- putative tumor suppressor gene can be very useful in detecting loss son, D., Kao, F.T. (1987). Hum. Genet. 76, 375-380. of heterozygosity in tumor samples. Acknowledgments: The authors thank Lois J. Maltais of The Jackson Laboratory for help in choosing the locus symbol that meets the nomecla- Identification and characterization of a ture guidelines. This research was supported by grants from National In- stitute of Health and Howard Hughes Medical Institute. A. Bradley is an microsatellite marker within murine Associate Investigator with HHMI. Brca2 gene References 1. Wooster, R., Bignell, G., Lancaster, J., Swift, S., Seal, S., Manglon, J., S.I~ Sharan, A. Bradley Collins, N., Gregory, S., Gumps, C., Micklems, G., Barfoot, R., Ham- oudi, R., Patel, S., Rice, C., Biggs, P., Hashim, Y., Smith, A., Connor, Howard Hughes Medical Institute, Department of Molecular and Human F., Arason, A., Gudmundsson, J., Ficenec, Kelsell, D., Ford, D., Tonin, Genetics, Baylor College of Medicine, One Baylor Plaza, P., Bishop, D.T., Spurt, N.K., Ponder, B.A., Eeles, R., Peto, J., Devilee, Houston, Texas 77030, USA P., Cornelisse, C., Lynch, H., Narod, S., Lenoir, G., Egilsson, V., Bark- adottir, R.B., Easton, D.F., Bentley, D.R., Futreal, P.A., Ashworth, A. Received: 28 August 1996 / Accepted: 5 September 1996 and Stratton, M.R. (1995). Nature 378, 789-792. 2. Tavtigan, S.V., Simard, J., Rommens, J., Couch, F., S.hattuck-Eidens, Species: Mus musculus D., Neuhausen, S., Merajver, S., Thorlacius, S., Offit, K., Stoppa- Locus symbol: D5Bayl Lyonnet, D., Belanger, C., Bell, R., Berry, S., Bogden, R., Chen, Q., Database deposit information: Genebank accession Number: Davis, T., Dumont, M., Frye, C., Hattier, T., Jammulapati, S., Janecki, U66168 T., Jiang, P., Kehrer, R., Leblanc, J.-F., Mitchell, J.T., McArthur- Molecular reagent: Forward primer, 5' CIT GTC TGG CAA Morrison, J., Nguyen, K., Peng, Y., Samson, C., Schroeder, M., Snyder, CAA ACG CA3'; reverse primer, 5' CAA GAG AAG GTG GTT S.C., Steele, L., Stringfellow, M., Stroup, C., Swedlund, B., Swensen, CAG AGY. B., Teng, D., Thomas, A., Tran, T., Tranchant, M., Weaver-Feldhaus, J., Wong, A.K.C., Shizuya, H., Eyfjord, J.E., Cannon-Albright, L., Labrie, Allele detection condition: PCR reaction: 50 ng of total genomic F., Skolnick, M.H., Weber, B., Kamb, A., and Goldgar, D.E. (1996). DNA, lxPCR buffer (Perkin Elmer), 1.0 mM MgCI2, 200 p.M Nature Genet. 12, 333-337. dNTPs, 1 I~M of each primer, and 2.5 units of Taq DNA polymer- ase in 50 i~1 total reaction volume. PCR conditions: 30 cycles of Using SSCP to facilitate mapping 94~ 1 rain; 55~ 1 min; 72~ 2 rain. Discussion: The microsatellite marker within the mouse homolog microsatellite loci of human breast and cancer susceptibility gene BRCA2 was iden- tified in an intron corresponding to human BRCA2 intron number J.L Williams, 1 I. Oisaker, 2 V.M. Teres 1 14 [1,2]. The microsatellite marker contains about 20 CA repeats (Fig. 1). Under the PCR conditions described above, the primers 1Division of Molecular Biology, Roslin Institute (Edinburgh), were used to amplifty 225 bp of mouse genomic DNA. The marker Midlothian, Scotland EH25 9PS, UK 2Department of Morphology, Genetics and Aquatic Biology, Division of Genetics, Norwegian College of Veterinary Medicine, P.O. Box 8146 Correspondence to: A. Bradley Dep. N-0033, Oslo, Norway Received: 15 July 1996 / Accepted: 21 August 1996 1 CCGGAGAGAG AGTACTTCAG TATGCTTGTC TGGCAACAAA CGCACGTACT Species: Bos tarus 51 GTGTGTGTGC GTGCATGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT Locus names: RI5 (D7S33), RI13 (D22S27), RI15 (D28S24), RI918 (D2S32) 101 GTGTGCGTGT GCGTGTGCCT GTGTGTTGAG AGACAGAGAT CAGGTGACTG Map position: See Table 1. 151 ATTTGCTCTT GTTAGCATTT ACACCACAGA ACGATACCAC AACATCCAAG Method of mapping: Segregation of alleles for the four markers was confirmed by typing the 347 individuals comprising 15 sire 201 GCTGTCGTTA TTCCTTACAA GTGTGGCCCC TCCCGCTCTG AACCACCTTC families of the International Bovine Reference Panel (IBRP) [1]. 251 TCTTGGCGAC CTGGAGGGGC TTACATGCTA GTAGCTCAGT CTTCTCTTCC Markers were assigned to bovine chromosomes by two-point link- 301 ATTCCAATCC CACCCGCCTT age analysis (CRIMAP). Molecular reagents: Oligonucleotide primer sequences are shown Fig. 1. Nucleotide sequence of D5Bayl. The underlined bases represent the polymorphic repeat present within the locus. in Table 1. For RI5, RI13, and RI15 sequences were obtained by http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Identification and characterization of a microsatellite marker within murine Brca2 gene

Free
1 page

Loading next page...
1 Page
 
/lp/springer_journal/identification-and-characterization-of-a-microsatellite-marker-within-O2SyOnZ6W0
Publisher
Springer-Verlag
Copyright
Copyright © 1997 by Springer-Verlag
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003359900623
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial