Hypoxia and inflammation in the release of VEGF and interleukins from human retinal pigment epithelial cells

Hypoxia and inflammation in the release of VEGF and interleukins from human retinal pigment... Graefes Arch Clin Exp Ophthalmol (2017) 255:1757–1762 DOI 10.1007/s00417-017-3711-0 BASIC SCIENCE Hypoxia and inflammation in the release of VEGF and interleukins from human retinal pigment epithelial cells 1 1,2 3,4 5 6,7 Olli Arjamaa & Vesa Aaltonen & Niina Piippo & Tamás Csont & Goran Petrovski & 4,8 3 Kai Kaarniranta & Anu Kauppinen Received: 26 January 2017 /Revised: 15 May 2017 /Accepted: 30 May 2017 /Published online: 19 June 2017 Springer-Verlag Berlin Heidelberg 2017 Abstract IL-8, IL-1β, and IL-18 were determined from medium sam- Purpose Retinal diseases are closely associated with both de- ples by an enzyme-linked immunosorbent assay (ELISA) creased oxygenation and increased inflammation. It is not method. Primary human RPE cells were exposed to hypoxia known if hypoxia-induced vascular endothelial growth factor for 24 h, and the subsequent release of IL-6 and IL-8 was (VEGF) expression in the retina itself evokes inflammation, or measured with ELISA. VEGF secretion from ARPE-19 cells whether inflammation is a prerequisite for the development of was determinedupto24h. neovascularization. Results Hypoxia induced significant (P < 0.01) increases in Methods Human ARPE-19 cell line and primary human reti- the levels of both IL-6 and IL-8 in ARPE-19 cells, and LPS nal pigment epithelium http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Graefe's Archive for Clinical and Experimental Ophthalmology Springer Journals

Hypoxia and inflammation in the release of VEGF and interleukins from human retinal pigment epithelial cells

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by Springer-Verlag Berlin Heidelberg
Subject
Medicine & Public Health; Ophthalmology
ISSN
0721-832X
eISSN
1435-702X
D.O.I.
10.1007/s00417-017-3711-0
Publisher site
See Article on Publisher Site

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