Graefes Arch Clin Exp Ophthalmol (2017) 255:1757–1762 DOI 10.1007/s00417-017-3711-0 BASIC SCIENCE Hypoxia and inflammation in the release of VEGF and interleukins from human retinal pigment epithelial cells 1 1,2 3,4 5 6,7 Olli Arjamaa & Vesa Aaltonen & Niina Piippo & Tamás Csont & Goran Petrovski & 4,8 3 Kai Kaarniranta & Anu Kauppinen Received: 26 January 2017 /Revised: 15 May 2017 /Accepted: 30 May 2017 /Published online: 19 June 2017 Springer-Verlag Berlin Heidelberg 2017 Abstract IL-8, IL-1β, and IL-18 were determined from medium sam- Purpose Retinal diseases are closely associated with both de- ples by an enzyme-linked immunosorbent assay (ELISA) creased oxygenation and increased inflammation. It is not method. Primary human RPE cells were exposed to hypoxia known if hypoxia-induced vascular endothelial growth factor for 24 h, and the subsequent release of IL-6 and IL-8 was (VEGF) expression in the retina itself evokes inflammation, or measured with ELISA. VEGF secretion from ARPE-19 cells whether inflammation is a prerequisite for the development of was determinedupto24h. neovascularization. Results Hypoxia induced significant (P < 0.01) increases in Methods Human ARPE-19 cell line and primary human reti- the levels of both IL-6 and IL-8 in ARPE-19 cells, and LPS nal pigment epithelium
Graefe's Archive for Clinical and Experimental Ophthalmology – Springer Journals
Published: Jun 19, 2017
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