Hypoxia and hydrogen cyanamide induce bud-break and up-regulate hypoxic responsive genes (HRG) and VvFT in grapevine-buds

Hypoxia and hydrogen cyanamide induce bud-break and up-regulate hypoxic responsive genes (HRG)... It has been reported that dormancy-breaking compound hydrogen cyanamide (HC) stimulates the fermentative pathway and inhibits respiration in grapevine-buds, suggesting in this way, that a respiratory stress must be involved in the release of buds from dormancy. Here, we tested low-oxygen effect (hypoxia) on the bud-break response of endodormant grapevine buds, and HC and hypoxia effects on the expression of hypoxic responsive genes (HRG) PYRUVATE DECARBOXYLASE (VvPDC), ALCOHOL DEHYDROGENASE (VvADH2), SUCROSE SYNTHASE (VvSUSY), non-symbiotic HEMOGLOBIN (VvnsHb), and on FLOWERING LOCUS T (VvFT), a transcription factor related to dormancy release in Vitis. Hypoxia as HC, induce transiently the expression of HRG and VvFT and hasten the sprouting of endodormant grapevine-buds. During the first 24 h after treatment, HRG and VvFT were strongly induced by hypoxia, subsequently, their expressions fell, and 14 days post-treatment increased again above control levels. These results indicate that in the short-term, a respiratory stress, caused either by oxygen deprivation or by inhibitors of respiration, induces transiently the expression of HRG and VvFT, and in the long-term, along with the advancement of bud-break, the expression of these genes move forward in treated buds, suggesting that these second induction that occurs just before bud-break is developmentally regulated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Hypoxia and hydrogen cyanamide induce bud-break and up-regulate hypoxic responsive genes (HRG) and VvFT in grapevine-buds

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Publisher
Springer Netherlands
Copyright
Copyright © 2012 by Springer Science+Business Media B.V.
Subject
Life Sciences; Plant Pathology; Biochemistry, general; Plant Sciences
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-012-9904-3
Publisher site
See Article on Publisher Site

Abstract

It has been reported that dormancy-breaking compound hydrogen cyanamide (HC) stimulates the fermentative pathway and inhibits respiration in grapevine-buds, suggesting in this way, that a respiratory stress must be involved in the release of buds from dormancy. Here, we tested low-oxygen effect (hypoxia) on the bud-break response of endodormant grapevine buds, and HC and hypoxia effects on the expression of hypoxic responsive genes (HRG) PYRUVATE DECARBOXYLASE (VvPDC), ALCOHOL DEHYDROGENASE (VvADH2), SUCROSE SYNTHASE (VvSUSY), non-symbiotic HEMOGLOBIN (VvnsHb), and on FLOWERING LOCUS T (VvFT), a transcription factor related to dormancy release in Vitis. Hypoxia as HC, induce transiently the expression of HRG and VvFT and hasten the sprouting of endodormant grapevine-buds. During the first 24 h after treatment, HRG and VvFT were strongly induced by hypoxia, subsequently, their expressions fell, and 14 days post-treatment increased again above control levels. These results indicate that in the short-term, a respiratory stress, caused either by oxygen deprivation or by inhibitors of respiration, induces transiently the expression of HRG and VvFT, and in the long-term, along with the advancement of bud-break, the expression of these genes move forward in treated buds, suggesting that these second induction that occurs just before bud-break is developmentally regulated.

Journal

Plant Molecular BiologySpringer Journals

Published: Mar 31, 2012

References

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