Water-soluble silicon nanoparticles (SiNPs) have been synthesized with photoluminescence quantum yield more than 32% via a hydrothermal treatment of 3-aminopropyltriethoxysilane as silicon sources and maleic acid (MA) as surface-stabilizing ligands. Prepared SiNPs showed the presence of carboxylic acid groups through the incorporation of MA. The presence of 48.8 and 51.2% of Si–Si and Si–O binding was observed in the resulting carboxylic acid-functionalized SiNPs (COOH-SiNPs). As revealed by the fluorescence lifetime images, COOH-SiNPs possesses several fluorophores mainly composed of above Si–Si binding inside of single particle, which explains the excitation-dependent fluorescence emission behavior of COOH-SiNPs. Also, the presence of oxides mainly composed of Si–O binding and MA on the surface of COOH-SiNPs provides long-term stability for both fluorescence and dispersion. The potential use of COOH-SiNPs as fluorescence bioimaging agents for cellular media has been demonstrated. COOH-SiNPs showed excellent cell viability more than 91% for both MDAMB and MDCK cells even in 1,000 ppm concentration, and multicolor fluorescence imaging (blue, green, and red) of MDAMB cells was successfully accomplished with different excitation wavelengths.
Journal of Materials Science – Springer Journals
Published: Nov 6, 2017
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