How to map ses, a mutant of Arabidopsis thaliana affecting pollen development

How to map ses, a mutant of Arabidopsis thaliana affecting pollen development In Arabidopsis, map-based cloning has been developed to an effective method in mutant genetic analysis because high-density markers are available, candidate genes or genomic sequences can be amplified by PCR, and transgenic techniques are simplified. Mutant ses named from shortened early-stage siliques was used as an example to show how to map a mutant in this way. By the process of bulked segregants analysis, linkage testing, large-scale and fine-scale mapping, mutant ses was narrowed into a 67 kb interval from CER448792 (2000541 bp) to CER464544 (2067844 bp) crossing over the right of BAC F12K11 to the left of the BAC F4H5 including at most 22 putative genes on the top of chromosome 1. In sequence-based map of Arabidopsis genes with mutant phenotype (SMAGMP) mutant ses was between AT1g06150 (EMB1444) and AT1g08060 (MOM). The ses mapping also showed that developed markers on polymorphism site of CAPC not only were simplified but worked well. Twenty-four markers from CAPC used in the mapping maybe help Arabidopsis researchs with others and the methods related to ses mapping also gave an example of positional cloning. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

How to map ses, a mutant of Arabidopsis thaliana affecting pollen development

Loading next page...
 
/lp/springer_journal/how-to-map-ses-a-mutant-of-arabidopsis-thaliana-affecting-pollen-I0Ke3ZqX8d
Publisher
Nauka/Interperiodica
Copyright
Copyright © 2006 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Microbial Genetics and Genomics; Animal Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795406080084
Publisher site
See Article on Publisher Site

Abstract

In Arabidopsis, map-based cloning has been developed to an effective method in mutant genetic analysis because high-density markers are available, candidate genes or genomic sequences can be amplified by PCR, and transgenic techniques are simplified. Mutant ses named from shortened early-stage siliques was used as an example to show how to map a mutant in this way. By the process of bulked segregants analysis, linkage testing, large-scale and fine-scale mapping, mutant ses was narrowed into a 67 kb interval from CER448792 (2000541 bp) to CER464544 (2067844 bp) crossing over the right of BAC F12K11 to the left of the BAC F4H5 including at most 22 putative genes on the top of chromosome 1. In sequence-based map of Arabidopsis genes with mutant phenotype (SMAGMP) mutant ses was between AT1g06150 (EMB1444) and AT1g08060 (MOM). The ses mapping also showed that developed markers on polymorphism site of CAPC not only were simplified but worked well. Twenty-four markers from CAPC used in the mapping maybe help Arabidopsis researchs with others and the methods related to ses mapping also gave an example of positional cloning.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Aug 17, 2006

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off