How Powerful is the Dwell-Time Analysis of Multichannel Records?

How Powerful is the Dwell-Time Analysis of Multichannel Records? Exact algorithms for the kinetic analysis of multichannel patch-clamp records require hours to days for a single record. Thus, it may be reasonable to use a fast but less accurate method for the analysis of all data sets and to use the results for a reanalysis of some selected records with more sophisticated approaches. For the first run, the tools of single-channel analysis were used for the evaluation of the single-channel rate constants from multichannel dwell-time histograms. This could be achieved by presenting an ensemble of single channels by a ``macrochannel'' comprising all possible states of the ensemble of channels. Equations for the calculations of the elements of the macrochannel transition matrix and for the steady-state concentrations for individual states are given. Simulations of multichannel records with 1 to 8 channels with two closed and one open states and with 2 channels with two open and two closed states were done in order to investigate under which conditions the one-dimensional dwell-time analysis itself already provides reliable results. Distributions of the evaluated single-channel rate constants show that a bias of the estimations of the single-channel rate constants of 10 to 20% has to be accepted. The comparison of simulations with signal-to-noise ratios of SNR = 1 or SNR = 25 demonstrates that the major problem is not the convergence of the fitting routine, but failures of the level detector algorithm which creates the dwell-times distributions from noisy time series. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

How Powerful is the Dwell-Time Analysis of Multichannel Records?

Loading next page...
 
/lp/springer_journal/how-powerful-is-the-dwell-time-analysis-of-multichannel-records-z5N743Z0dR
Publisher
Springer-Verlag
Copyright
Copyright © Inc. by 1998 Springer-Verlag New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s002329900417
Publisher site
See Article on Publisher Site

Abstract

Exact algorithms for the kinetic analysis of multichannel patch-clamp records require hours to days for a single record. Thus, it may be reasonable to use a fast but less accurate method for the analysis of all data sets and to use the results for a reanalysis of some selected records with more sophisticated approaches. For the first run, the tools of single-channel analysis were used for the evaluation of the single-channel rate constants from multichannel dwell-time histograms. This could be achieved by presenting an ensemble of single channels by a ``macrochannel'' comprising all possible states of the ensemble of channels. Equations for the calculations of the elements of the macrochannel transition matrix and for the steady-state concentrations for individual states are given. Simulations of multichannel records with 1 to 8 channels with two closed and one open states and with 2 channels with two open and two closed states were done in order to investigate under which conditions the one-dimensional dwell-time analysis itself already provides reliable results. Distributions of the evaluated single-channel rate constants show that a bias of the estimations of the single-channel rate constants of 10 to 20% has to be accepted. The comparison of simulations with signal-to-noise ratios of SNR = 1 or SNR = 25 demonstrates that the major problem is not the convergence of the fitting routine, but failures of the level detector algorithm which creates the dwell-times distributions from noisy time series.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Sep 1, 1998

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off