Access the full text.
Sign up today, get DeepDyve free for 14 days.
A. Gatignol, Charles Buckler, K. Jeang (1993)
Relatedness of an RNA-binding motif in human immunodeficiency virus type 1 TAR RNA-binding protein TRBP to human P1/dsI kinase and Drosophila staufenMolecular and Cellular Biology, 13
Jonathan Pollack, Don Ganem (1993)
An RNA stem-loop structure directs hepatitis B virus genomic RNA encapsidationJournal of Virology, 67
S. Kim, Hakdong Shin, Y. Cho, Hyune Rho (2000)
Expression of stable hepatitis B viral polymerase associated with GRP94 in E. coliArchives of Virology, 145
J. Melnick, J. Dul, Y. Argon (1994)
Sequential interaction of the chaperones BiP and GRP94 with immunoglobulin chains in the endoplasmic reticulumNature, 370
R. Lanford, L. Notvall, B. Beames (1995)
Nucleotide priming and reverse transcriptase activity of hepatitis B virus polymerase expressed in insect cellsJournal of Virology, 69
R. Bartenschlager, M. Junker-Niepmann, H. Schaller (1990)
The P gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidationJournal of Virology, 64
G. Koch, M. Smith, D. Macer, P. Webster, R. Mortara (1986)
Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin.Journal of cell science, 86
B. Corthésy, P. Kao (1994)
Purification by DNA affinity chromatography of two polypeptides that contact the NF-AT DNA binding site in the interleukin 2 promoter.The Journal of biological chemistry, 269 32
D. Ganem, H. Varmus (1987)
The molecular biology of the hepatitis B viruses.Annual review of biochemistry, 56
R. Hirsch, J. Lavine, Lung-Ji Chang, H. Varmus, D. Ganem (1990)
Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as well as for reverse transcriptionNature, 344
H. Shin, HM Rho (1995)
Release of the hepatitis B virus-associated DNA polymerase from the viral particle by the proteolytic cleavageThe Journal of Biological Chemistry, 270
(1997)
DO,Seeger, C (1997)Hepadnavirus assembly and reverse transcription require a multicomponent chaperone complex which is incorporated into nucleocapsids
J. Tavis, Silvia Perri, Don Ganem (1994)
Hepadnavirus reverse transcription initiates within the stem-loop of the RNA packaging signal and employs a novel strand transferJournal of Virology, 68
E. Little, Meera Ramakrishnan, Binayak Roy, G. Gazit, Amy Lee (1994)
The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications.Critical reviews in eukaryotic gene expression, 4 1
A. Rieger, M. Nassal (1996)
Specific hepatitis B virus minus-strand DNA synthesis requires only the 5' encapsidation signal and the 3'-proximal direct repeat DR1Journal of Virology, 70
H. Rho, R. Gallo (1980)
Biochemical and immunological properties of the DNA polymerase and RNAase H activities of purified feline leukemia virus reverse transcriptase.Cancer letters, 10 3
P. Tiollais, P. Charnay, G. Vyas (1981)
Biology of hepatitis B virus.Science, 213 4506
T. Nieland, M. Tan, M. Muijen, F. Koning, A. Kruisbeek, G. Bleek (1996)
Isolation of an immunodominant viral peptide that is endogenously bound to the stress protein GP96/GRP94.Proceedings of the National Academy of Sciences of the United States of America, 93 12
J. Dignam, R. Lebovitz, R. Roeder (1983)
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.Nucleic acids research, 11 5
W. Schaiff, Keith Hruska, David Mccourt, Michad Green, Benjamin Schwartz (1992)
HLA-DR associates with specific stress proteins and is retained in the endoplasmic reticulum in invariant chain negative cellsThe Journal of Experimental Medicine, 176
M. Nassal, A. Rieger (1996)
A bulged region of the hepatitis B virus RNA encapsidation signal contains the replication origin for discontinuous first-strand DNA synthesisJournal of Virology, 70
J. Tavis, D. Ganem (1993)
Expression of functional hepatitis B virus polymerase in yeast reveals it to be the sole viral protein required for correct initiation of reverse transcription.Proceedings of the National Academy of Sciences of the United States of America, 90
R. Mazzarella, M. Green (1987)
ERp99, an abundant, conserved glycoprotein of the endoplasmic reticulum, is homologous to the 90-kDa heat shock protein (hsp90) and the 94-kDa glucose regulated protein (GRP94).The Journal of biological chemistry, 262 18
E. Helgstrand, B. Eriksson, N. Johansson, B. Lannerö, A. Larsson, A. Misiorny, J. Noren, B. Sjöberg, K. Stenberg, G. Stening, S. Stridh, B. Oberg (1978)
Trisodium phosphonoformate, a new antiviral compound.Science, 201 4358
Jianming Hu, C. Seeger (1996)
Hsp90 is required for the activity of a hepatitis B virus reverse transcriptase.Proceedings of the National Academy of Sciences of the United States of America, 93 3
'. JONATHANR.POLLACK, Don Ganem (1994)
Site-specific RNA binding by a hepatitis B virus reverse transcriptase initiates two distinct reactions: RNA packaging and DNA synthesisJournal of Virology, 68
Peter Kao, Lei Chen, G. Brock, J. Ng, J. Kenny, A. Smith, Blaise Corthésy (1994)
Cloning and expression of cyclosporin A- and FK506-sensitive nuclear factor of activated T-cells: NF45 and NF90.The Journal of biological chemistry, 269 32
Hwai-Wen Chang, Bertram Jacobs (1993)
Identification of a conserved motif that is necessary for binding of the vaccinia virus E3L gene products to double-stranded RNA.Virology, 194 2
Guang-hua Wang, C. Seeger (1992)
The reverse transcriptase of hepatitis B virus acts as a protein primer for viral DNA synthesisCell, 71
(1998)
DNA-dependent protein kinase interactswith antigen receptor responseelement bindingproteinsNF90andNF45
David Navarro, Pedro Paz, Lenore Pereira (1992)
Domains of herpes simplex virus I glycoprotein B that function in virus penetration, cell-to-cell spread, and cell fusion.Virology, 186 1
N. Ting, P. Kao, D. Chan, L. Lintott, S. Lees-Miller (1998)
DNA-dependent Protein Kinase Interacts with Antigen Receptor Response Element Binding Proteins NF90 and NF45*The Journal of Biological Chemistry, 273
M. Seifer, N. David, STANDRINGl (1993)
Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the nucleocapsid or the viral replication origin, DR1Journal of Virology, 67
Dorothy Fallows, S. Goff (1995)
Mutations in the epsilon sequences of human hepatitis B virus affect both RNA encapsidation and reverse transcriptionJournal of Virology, 69
M. Junker-Niepmann, R. Bartenschlager, H. Schaller (1990)
A short cis‐acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA.The EMBO Journal, 9
Jianming Hu, D. Toft, C. Seeger (1997)
Hepadnavirus assembly and reverse transcription require a multi‐component chaperone complex which is incorporated into nucleocapsidsThe EMBO Journal, 16
R. Hirsch, D. Loeb, J. Pollack, D. Ganem (1991)
cis-acting sequences required for encapsidation of duck hepatitis B virus pregenomic RNAJournal of Virology, 65
H. Rho, K. Kim, S. Hyun, Y. Kim (1989)
The nucleotide sequence and reading frames of a mutant hepatitis B virus subtype adr.Nucleic acids research, 17 5
Silvia Perri, Don Ganem (1996)
A host factor that binds near the termini of hepatitis B virus pregenomic RNAJournal of Virology, 70
Martin Weber, Viola Bronsema, H. Bartos, A. Bosserhoff, R. Bartenschlager, H. Schaller (1994)
Hepadnavirus P protein utilizes a tyrosine residue in the TP domain to prime reverse transcriptionJournal of Virology, 68
E. Lara-Pezzi, Angel Armesilla, P. Majano, Juan Redondo, M. López-Cabrera (1998)
The hepatitis B virus X protein activates nuclear factor of activated T cells (NF‐AT) by a cyclosporin A‐sensitive pathwayThe EMBO Journal, 17
Guang-Hu Wang, F. Zoulim, E. Leber, J. Kitson, C. Seeger (1994)
Role of RNA in enzymatic activity of the reverse transcriptase of hepatitis B virusesJournal of Virology, 68
M. Sells, M. Chen, G. Acs (1987)
Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA.Proceedings of the National Academy of Sciences of the United States of America, 84 4
R. Suto, P. Srivastava (1995)
A mechanism for the specific immunogenicity of heat shock protein-chaperoned peptides.Science, 269 5230
R. Bartenschlager, H. Schaller (1992)
Hepadnaviral assembly is initiated by polymerase binding to the encapsidation signal in the viral RNA genome.The EMBO Journal, 11
Guang-hua Wang, C. Seeger (1993)
Novel mechanism for reverse transcription in hepatitis B virusesJournal of Virology, 67
Zihai Li, P. Srivastava (1993)
Tumor rejection antigen gp96/grp94 is an ATPase: implications for protein folding and antigen presentation.The EMBO Journal, 12
le Arnold, S. Faath, H. Rammensee, rg Schild (1995)
Cross-priming of minor histocompatibility antigen-specific cytotoxic T cells upon immunization with the heat shock protein gp96The Journal of Experimental Medicine, 182
F. Zoulim, C. Seeger (1994)
Reverse transcription in hepatitis B viruses is primed by a tyrosine residue of the polymeraseJournal of Virology, 68
Meera Ramakrishnan, Sharof Tugizov, Lenore Pereira, Amy Lee (1995)
Conformation-defective herpes simplex virus 1 glycoprotein B activates the promoter of the grp94 gene that codes for the 94-kD stress protein in the endoplasmic reticulum.DNA and cell biology, 14 5
C. Chavany, E. Mimnaugh, P. Miller, R. Bitton, P. Nguyen, J. Trepel, L. Whitesell, R. Schnur, J. Moyer, L. Neckers (1996)
p185erbB2 binds to GRP94 in vivo. Dissociation of the p185erbB2/GRP94 heterocomplex by benzoquinone ansamycins precedes depletion of p185erbB2.The Journal of biological chemistry, 271 9
E. Little, Amy Lee (1995)
Generation of a Mammalian Cell Line Deficient in Glucose-regulated Protein Stress Induction through Targeted Ribozyme Driven by a Stress-inducible Promoter (*)The Journal of Biological Chemistry, 270
D. Johnston, N. Brown, J. Gallt, Michael JANTSCHt (1992)
A conserved double-stranded RNA-binding domain.Proceedings of the National Academy of Sciences of the United States of America, 89 22
Summary. The highly conserved encapsidation signal (ɛ) of hepatitis B viral (HBV) pregenomic RNA has been reported as an essential component for encapsidation and protein priming of HBV polymerase. Here, we report that two HBV ɛ RNA-binding host proteins (80 and 43 kDa) and a copurifying protein (100 kDa) were purified and characterized by the combined methods of UV cross-linking analysis with the ɛ RNA and column chromatography. Amino-terminal micro-sequencing showed that 80- and 43-kDa proteins were identified as the heterodimeric nuclear factor of activated T cells (NF90/NF45) and 100 kDa as a molecular chaperone, the GRP94. The heterodimeric factor interacted preferentially with the upper-bulge region of HBV ɛ RNA helping the HBV polymerase bind the lower-bulge region. Using in vitro protein priming analysis, the initial oligonucleotide of the protein-priming product was deduced as 5′-GAAC-3′, which is the complementary sequence of both regions of DR1 and ɛ in the pregenomic RNA. Previously, we also proposed that the GRP94 was associated with HBV polymerase in the human liver cell HepG2. These results suggest that the heterodimeric factor plays an important role in the priming activity of HBV polymerase.
Archives of Virology – Springer Journals
Published: Mar 1, 2002
Keywords: Cell HepG2; Molecular Chaperone; Host Protein; Priming Activity; Complementary Sequence
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.