It is common knowledge that zebrafish, Danio rerio, oocytes in their follicular envelope that have reached definitive size undergo in vitro maturation in 90% Leibovitz’s medium, pH 9.0, when treated with 17α, 20β-dihydroxyprogesterone and acquire developmental competence but do not ovulate (Seki et al., 2008). We have demonstrated that zebrafish oocytes that have undergone maturation under the indicated conditions ovulate when treated with prostaglandin F2α (5 μg/mL) and/or 20% carp ovarial fluid and are capable of development towards the actively feeding larvae upon fertilization (the maximum follow-up period).
Russian Journal of Developmental Biology – Springer Journals
Published: Sep 24, 2016
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