Hormone-induced in vitro maturation and ovulation of Danio rerio oocytes and production of eggs capable of fertilization and futher development

Hormone-induced in vitro maturation and ovulation of Danio rerio oocytes and production of eggs... It is common knowledge that zebrafish, Danio rerio, oocytes in their follicular envelope that have reached definitive size undergo in vitro maturation in 90% Leibovitz’s medium, pH 9.0, when treated with 17α, 20β-dihydroxyprogesterone and acquire developmental competence but do not ovulate (Seki et al., 2008). We have demonstrated that zebrafish oocytes that have undergone maturation under the indicated conditions ovulate when treated with prostaglandin F2α (5 μg/mL) and/or 20% carp ovarial fluid and are capable of development towards the actively feeding larvae upon fertilization (the maximum follow-up period). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

Hormone-induced in vitro maturation and ovulation of Danio rerio oocytes and production of eggs capable of fertilization and futher development

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Publisher
Pleiades Publishing
Copyright
Copyright © 2016 by Pleiades Publishing, Inc.
Subject
Life Sciences; Developmental Biology; Animal Anatomy / Morphology / Histology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S106236041605009X
Publisher site
See Article on Publisher Site

Abstract

It is common knowledge that zebrafish, Danio rerio, oocytes in their follicular envelope that have reached definitive size undergo in vitro maturation in 90% Leibovitz’s medium, pH 9.0, when treated with 17α, 20β-dihydroxyprogesterone and acquire developmental competence but do not ovulate (Seki et al., 2008). We have demonstrated that zebrafish oocytes that have undergone maturation under the indicated conditions ovulate when treated with prostaglandin F2α (5 μg/mL) and/or 20% carp ovarial fluid and are capable of development towards the actively feeding larvae upon fertilization (the maximum follow-up period).

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Sep 24, 2016

References

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