Hormonal induction of in vitro maturation and ovulation of loach oocytes and obtaining egg cells capable of fertilization and development

Hormonal induction of in vitro maturation and ovulation of loach oocytes and obtaining egg cells... Loach oocytes that have reached a definitive size and are surrounded by follicular envelopes are capable of maturation and ovulating under the effect of 1 μg/mL progesterone in 75% Leibovitz medium with 1 g/L sodium bicarbonate or with pH adjustment to 9.0 by 1 N sodium hydroxide. Inseminated eggs are developed until the stage of prelarvae when adding 20% bovine serum to the incubation medium. Substitution of the bovine serum with 10–20% loach ovarian fluid or 20% carp ovarian fluid provides more complete development of inseminated eggs until the stage of larva that pass to active feeding. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

Hormonal induction of in vitro maturation and ovulation of loach oocytes and obtaining egg cells capable of fertilization and development

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Inc.
Subject
Life Sciences; Developmental Biology; Animal Anatomy / Morphology / Histology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360415030078
Publisher site
See Article on Publisher Site

Abstract

Loach oocytes that have reached a definitive size and are surrounded by follicular envelopes are capable of maturation and ovulating under the effect of 1 μg/mL progesterone in 75% Leibovitz medium with 1 g/L sodium bicarbonate or with pH adjustment to 9.0 by 1 N sodium hydroxide. Inseminated eggs are developed until the stage of prelarvae when adding 20% bovine serum to the incubation medium. Substitution of the bovine serum with 10–20% loach ovarian fluid or 20% carp ovarian fluid provides more complete development of inseminated eggs until the stage of larva that pass to active feeding.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: May 26, 2015

References

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