Homologous and heterologous protein import into mitochondria isolated from the green alga Chlamydomonas reinhardtii

Homologous and heterologous protein import into mitochondria isolated from the green alga... We have established a homologous system for studying mitochondrial protein import in Chlamydomonas reinhardtii, using C. reinhardtii precursor proteins and mitochondria isolated from C. reinhardtii. The precursors of the F1α ATP synthase subunit and the Rieske FeS protein were imported into mitochondria with high efficiency, while the F1β subunit precursor was imported with much lower efficiency. The import of heterologous precursor proteins from higher plants was also less efficient. The precursor of the C. reinhardtii PsaF chloroplast protein was converted into a protease-protected form upon incubation with mitochondria. In vitro processing studies revealed that in contrast to the situation in higher plants, the processing of the precursors was catalysed by a soluble, matrix-located peptidase. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Homologous and heterologous protein import into mitochondria isolated from the green alga Chlamydomonas reinhardtii

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1997 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005878614878
Publisher site
See Article on Publisher Site

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