Hirame rhabdovirus (HIRRV) as the cause of a natural disease outbreak in cultured black seabream ( Acanthopagrus schlegeli ) in Korea

Hirame rhabdovirus (HIRRV) as the cause of a natural disease outbreak in cultured black seabream... In 2015, a high mortality rate of about 40 % was observed in black seabream ( Acanthopagrus schlegeli ) on a farm on the southern coast of Korea. Most of the diseased fish showed a hemorrhage of the mouth, pale liver, petechial hemorrhaging in the internal fat, and an enlarged spleen. Other than Alella sp., no parasites or bacteria were isolated from the diseased fish, and all of the tissue filtrates produced cytopathic effects (CPEs) in FHM and CHSE-214 cells. A polymerase chain reaction analysis revealed that the cell culture supernatants with CPE expressed specific 730-bp fragments for the hirame rhabdovirus (HIRRV) phosphoprotein gene. The nucleotide sequences showed a minimum of 95.8 % identity to five other known isolates of HIRRV, including CA-9703 and 8401-H from olive flounder ( Paralichthys olivaceus ) in Korea and Japan. An experimental challenge was conducted in which the virus was delivered by injection, and the cumulative mortalities of black seabream challenged with this new HIRRV isolate at 10 4.8 TCID 50 /fish and 10 3.8 TCID 50 /fish were 100 % and 20 %, respectively. This fulfilled Koch’s postulates and confirmed that HIRRV was the cause of disease and mortality for both the natural and experimental infection of black seabream. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Hirame rhabdovirus (HIRRV) as the cause of a natural disease outbreak in cultured black seabream ( Acanthopagrus schlegeli ) in Korea

Loading next page...
 
/lp/springer_journal/hirame-rhabdovirus-hirrv-as-the-cause-of-a-natural-disease-outbreak-in-o0z4BJ0BWF
Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2573-1
Publisher site
See Article on Publisher Site

Abstract

In 2015, a high mortality rate of about 40 % was observed in black seabream ( Acanthopagrus schlegeli ) on a farm on the southern coast of Korea. Most of the diseased fish showed a hemorrhage of the mouth, pale liver, petechial hemorrhaging in the internal fat, and an enlarged spleen. Other than Alella sp., no parasites or bacteria were isolated from the diseased fish, and all of the tissue filtrates produced cytopathic effects (CPEs) in FHM and CHSE-214 cells. A polymerase chain reaction analysis revealed that the cell culture supernatants with CPE expressed specific 730-bp fragments for the hirame rhabdovirus (HIRRV) phosphoprotein gene. The nucleotide sequences showed a minimum of 95.8 % identity to five other known isolates of HIRRV, including CA-9703 and 8401-H from olive flounder ( Paralichthys olivaceus ) in Korea and Japan. An experimental challenge was conducted in which the virus was delivered by injection, and the cumulative mortalities of black seabream challenged with this new HIRRV isolate at 10 4.8 TCID 50 /fish and 10 3.8 TCID 50 /fish were 100 % and 20 %, respectively. This fulfilled Koch’s postulates and confirmed that HIRRV was the cause of disease and mortality for both the natural and experimental infection of black seabream.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2015

References

  • Complete nucleotide sequence of the hirame rhabdovirus, a pathogen of marine fish
    Kim, DH; Oh, HK; Eou, JI; Seo, HJ; Kim, SK; Oh, MJ; Nam, SW; Choi, TJ

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off