High-yield production of canine parvovirus virus-like particles in a baculovirus expression system

High-yield production of canine parvovirus virus-like particles in a baculovirus expression system An optimized VP2 gene from the current prevalent CPV strain (new CPV-2a) in China was expressed in a baculovirus expression system. It was found that the VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and with an especially high hemagglutination (HA) titer (1:2 20 ). Dogs intramuscularly or orally immunized with VLPs produced antibodies against CPV with >1:80 hemagglutination inhibition (HI) units for at least 3 months. The CPV VLPs could be considered for use as a vaccine against CPV or as a platform for research on chimeric VLP vaccines against other diseases. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

High-yield production of canine parvovirus virus-like particles in a baculovirus expression system

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2719-1
Publisher site
See Article on Publisher Site

Abstract

An optimized VP2 gene from the current prevalent CPV strain (new CPV-2a) in China was expressed in a baculovirus expression system. It was found that the VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and with an especially high hemagglutination (HA) titer (1:2 20 ). Dogs intramuscularly or orally immunized with VLPs produced antibodies against CPV with >1:80 hemagglutination inhibition (HI) units for at least 3 months. The CPV VLPs could be considered for use as a vaccine against CPV or as a platform for research on chimeric VLP vaccines against other diseases.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2016

References

  • Molecular characterization of canine parvovirus strains in Argentina: detection of the pathogenic variant CPV2c in vaccinated dogs
    Calderon, MG; Mattion, N; Bucafusco, D; Fogel, F; Remorini, P; Torre, J
  • Use of parvovirus-like particles for vaccination and induction of multiple immune responses
    Casal, JI
  • Recombinant ELISA using baculovirus-expressed VP2 for detection of antibodies against canine parvovirus
    Elia, G; Desario, C; Pezzoni, G; Camero, M; Brocchi, E; Decaro, N; Martella, V; Buonavoglia, C
  • Multimerization of peptide antigens for production of stable immunogens in transgenic plants
    Gil, F; Reytor, E; Perez-Filgueira, DM; Escribano, JM
  • Phylogenetic analysis of the VP2 gene of canine parvoviruses circulating in China
    Zhang, R; Yang, S; Zhang, W; Zhang, T; Xie, Z; Feng, H; Wang, S; Xia, X

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