Heterotrimeric G-proteins are involved in a variety of cellular responses, but relatively little is known about their function and biochemistry in plants. Antibodies raised against the tobacco heterotrimeric G-protein β-subunit (Gβ) were used to analyse its distribution in tobacco leaves. In young tissue the protein level was relatively high, while it declined substantially during later stages of leaf development. Cell fractionation revealed that Gβ is tightly associated with plasma membrane, but can also be detected in purified nuclei.
Plant Molecular Biology – Springer Journals
Published: Oct 16, 2004
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