Hepatoprotective and hematoprotective effects of Falcaria vulgaris
aqueous extract against CCl
-induced hepatic injury in mice
Mohammad Mahdi Zangeneh
Received: 6 February 2018 /Accepted: 17 May 2018
Springer-Verlag London Ltd., part of Springer Nature 2018
Medicinal plants are considered as modern resources for producing agents that could act as alternatives to chemical drugs.
Falcaria vulgaris (FV) has been used in medicine as an antioxidant, antifungal, anti-inflammatory, and antibacterial agent.
The aim of the present study was to investigate the hepatoprotective and hematoprotective effects of FV aqueous extract against
-induced hepatotoxicity in mice. Sixty male mice were divided into six groups (n = 10); group I served as control, received
1 mL/kg olive oil intraperitoneally and 0.5 mL distilled water through gavage. Group II served as untreated group, received 1 mg/
mixed with olive oil in the ratio of 5:5 intraperitoneally and 0.5 mL distilled water orally. Groups III, IV, V, and VI
mixed with olive oil in the ratio of 5:5 intraperitoneally and 20, 40, 80, and 160 mg/kg of FV aqueous extract
through gavage for 45 consecutive days. Different doses of FV (especially FV160) could significantly (p ≤ 0.05) decrease the
raised levels of alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, cholesterol, low-density lipoprotein,
white blood cell, and platelet and increase high-density lipoprotein, superoxide dismutase, catalase, and red blood cell as
compared to the untreated group. The weight and volume of the hepatic structures were decreased significantly (p ≤ 0.05) in
different doses of FV (especially FV160) compared to the untreated group. FV aqueous extract can protect hepatic tissue and
regulate liver enzymes in CCl
-induced hepatotoxicity. FV has hepatoprotective and hematoprotective properties, thereby re-
ducing the causation of diabetes in experimental mice.
Keywords Falcaria vulgaris
Carbon tetrachloride (CCl
) is a toxic substance for most organs
of the body such as the liver, kidneys, heart, testes, brain, and
blood (Alia et al. 2003). Furthermore, different documented
case studies have established that CCl
produces hepatic disease
with a changed antioxidant status in humans (Alia et al. 2003).
Findings from the screening of several ethno medicinal plants
describe their antioxidant effect and demonstrate that they could
protect the liver against CCl
by increasing the levels of antiox-
idant enzymes (Goodarzi et al. 2017; Sherkatolabbasieh et al.
2017). Research on hepatoprotective agents is one of the ad-
vanced fields in recent biomedical sciences. The expensive
costs of modern medicines indicate that alternative strategies
are need for better management of diseases and their related
Ethno medicinal plants are popular remedies used by most
people (Faramarzi et al. 2017; Foroughi et al. 2016; Tahvilian
et al. 2017). The impression of ethno medicinal plants in pre-
vention and treatment of diseases is irrecusable (Najafi et al.
2017). Aqueous extract is a kind of fraction of plants that in-
cludes aromatic and nonaromatic compounds (Moradi et al.
2017). This fraction could be extracted from several parts such
as fruits, flowers, leaves, stems, and roots (Poorshamohammad
et al. 2017). In recent years, interest in aqueous extract has been
* Mohammad Mahdi Zangeneh
Department of Clinical Science, Faculty of Veterinary Medicine,
Razi University, Kermanshah, Iran
Pharmaceutical Sciences Research Center, Faculty of Pharmacy,
Kermanshah University of Medical Sciences, Kermanshah, Iran
Department of Basic Science, Faculty of Veterinary Medicine, Razi
University, Kermanshah, Iran
Substance Abuse Prevention Research Center, Kermanshah
University of Medical Sciences, Kermanshah, Iran
Comparative Clinical Pathology