Helicoverpa armigera nucleopolyhedrovirus orf81 is a late gene involved in budded virus production

Helicoverpa armigera nucleopolyhedrovirus orf81 is a late gene involved in budded virus production Helicoverpa armigera nucleopolyhedrovirus (HearNPV) orf81 ( ha81 ) is a core gene that is highly conserved in all lepidopteran baculoviruses. Its homolog in the group I baculoviruses, ac93 , has been shown to be essential for the nuclear egress of nucleocapsids, but its role in the group II HearNPV life cycle remains unknown. In this study, an ha81 mutant bacmid was constructed by homologous recombination to investigate the role of HA81 in the viral life cycle. Quantitative PCR analysis showed that viral DNA replication was unaffected in the absence of ha81 . However, the budded virus production of the ha81 -null virus was completely blocked. Transmission electron microscopic analysis showed that ha81 is required for the egress of nucleocapsids from the nucleus. Analysis of the time course of transcription and expression revealed that ha81 is a late gene. An immunofluorescence analysis showed that the protein mainly localizes in the cytoplasm. To understand whether the transcription of other genes is affected by the deletion of ha81 , the transcription of several well-characterized viral genes was investigated in the ha81 -knockout HearNPV mutant. No obvious changes were observed at the transcription level, except for the odv-e25 gene downstream from ha81 . In conclusion, these data indicate that ha81 is a late gene that is critical for budded virus production but is involved in neither viral DNA replication nor gene transcription. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Helicoverpa armigera nucleopolyhedrovirus orf81 is a late gene involved in budded virus production

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2034-2
Publisher site
See Article on Publisher Site

References

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    Wang, H; Deng, F; Pijlman, GP; Chen, X; Sun, X; Vlak, JM; Hu, Z
  • Characterization of Helicoverpa armigera nucleopolyhedrovirus orf33 that encodes a novel budded virion derived protein, BV-e31
    Wang, D; An, SH; Guo, ZJ; Xu, HJ; Zhang, CX
  • HearSNPV orf83 encodes a late, nonstructural protein with an active chitin-binding domain
    Wang, D; Zhang, CX
  • Autographa californica multiple nucleopolyhedrovirus odv-e25 (Ac94) is required for budded virus infectivity and occlusion-derived virus formation
    Chen, L; Hu, X; Xiang, X; Yu, S; Yang, R; Wu, X
  • Identification and characterization of odv-e25 of Spodoptera litura multicapsid nucleopolyhedrovirus
    Li, Z; Pan, L; Yu, H; Li, S; Zhang, G; Pang, Y

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