1063-0740/02/2802- $27.00 © 2002
Russian Journal of Marine Biology, Vol. 28, No. 2, 2002, pp. 113–119.
Original Russian Text Copyright © 2002 by Biologiya Morya, Kovekovdova, Simokon’.
Data about the distribution of microelements in
commercially important ﬁsh of the World Ocean are of
great theoretical and practical interest. They expand our
knowledge about the biological role of microelements
and they provide information about the quality of
marine food sources.
The coastal ﬁshery zones in Peter the Great Bay are
polluted by many substances, including heavy metals
[15, 18]. There are many papers dealing with heavy
metal concentrations in marine hydrobionts; however,
marine ﬁsh in Peter the Great Bay still remain relatively
poorly studied. Among the second-order bays belong-
ing to the Peter the Great Bay system, Amurskii Bay is
one of the most heavily polluted. Thus, the goal of our
study is as follows: to determine the concentrations of
speciﬁc heavy metals (iron, copper, zinc, manganese,
cadmium, and mercury) in the tissue of the most com-
mon commercially important ﬁsh species inhabiting
Amurskii Bay, namely Paciﬁc herring, Far Eastern nav-
aga, and spotted ﬂounder. Our goals also included the
study of the distribution of these elements in organs and
tissues of the ﬁsh and an evaluation of their sanitary and
hygienic conditions. This information about the distri-
bution of the elements in organs and tissues of the
examined ﬁsh species should extend our knowledge
about their biological role and reveal species-speciﬁc
patterns of heavy metal accumulation.
MATERIALS AND METHODS
We studied Paciﬁc herring (
Clupea pallasi pallasi
) and spotted ﬂounder
) that were sampled from under
the ice using a stand net in Amurskii Bay in the area of
Sedanka railway station (in the suburbs of Vladivostok)
in March 1994. To determine the wet weight, we
weighed 12–16 specimens of each species that were
approximately the same size. The ﬁsh were then dis-
sected. Organs and tissues (muscles, liver, gills, skin,
and bones) were isolated and also weighed. For all sam-
ples, we determined the percentage of water concentra-
tion in the organs and tissues using standard techniques.
The preparation of the samples for atom-absorption
analysis was performed using the method of acid min-
eralization according to GOST 26929-94. The list of
elements to be determined included zinc, iron, copper,
cadmium, manganese, and mercury. Among these ele-
ments, iron, manganese, copper, and zinc are referred
to as “true bioelements.” Mercury and cadmium, on the
other hand, are the most characteristic representatives
of technogenic metals, and they occupy top positions in
all lists of toxicants.
The samples for concentrations of zinc, iron, cop-
per, cadmium, and manganese were analyzed by the
atom-absorption spectrophotometry method with a
Nippon Jarrell Ash spectrophotometer (model AA-855)
. The mercury content was determined using ﬂame-
less atom-absorption spectrophotometry with a
Hiranuma Hg-1 device. For comparison, we used stan-
dard laboratory samples of metal solutions listed in the
Federal Register of Measuring Tools. The relative mean
error did not exceed 5%.
The total number of tests performed to determine
the concentrations of the metals equaled 1440.
Heavy Metals in the Tissues of Commercially Important Fish
of Amurskii Bay, Sea of Japan
L. T. Kovekovdova and M. V. Simokon’
Paciﬁc Center of Scientiﬁc Fisheries, Vladivostok, 690600 Russia
Received September 26, 2001
—The concentrations of iron, copper, zinc, manganese, cadmium, and mercury in tissues and organs
of Paciﬁc herring, Far Eastern navaga, and spotted ﬂounder from Amurskii Bay, Sea of Japan, were determined
using an atom-absorption method. The distribution of these elements has been studied in organisms of the ﬁsh.
The greatest concentration of iron, copper, cadmium, and mercury is found in the liver of the ﬁsh, manganese
is mostly accumulated in the bone tissue, and zinc is found in the skin. Some speciﬁc features of metal accu-
mulation in the ﬁsh of Amurskii Bay have been revealed. For example, the concentration of iron in the liver of
herring and ﬂounder signiﬁcantly increased the mean concentration known from other areas. A sanitary–
hygienic evaluation is provided for the recent levels of metal concentrations in these three species of commer-
cially important ﬁsh.
heavy metals, commercially important ﬁsh, atom-absorption analysis.