HBx and HBs regulate RhoC expression by upregulating transcription factor Ets-1

HBx and HBs regulate RhoC expression by upregulating transcription factor Ets-1 The available evidence suggests that HBV proteins play an important role in the development of hepatocellular carcinoma (HCC). RhoC, a member of the Rho subfamily of the Ras superfamily of homologous genes, had been implicated in tumorigenesis and tumor progression. In a previous study, we demonstrated that HBx and HBs could up-regulate RhoC expression by enhancing its promoter activity. However, the specific mechanisms remain unclear. Here, we demonstrate that overexpression of Ets-1 results in upregulation of RhoC promoter activity and mRNA and protein levels. Expression of transcription factor Ets-1 was significantly higher in HepG2.2.15 cells than that in HepG2 cells. Meanwhile, infection of HepG2 cells with an HBV-adenovirus recombinant virus led to up-regulation of Ets-1. Of the four HBV proteins, HBx and HBs, could increase expression of Ets-1, which consequently contributed to the upregulation of RhoC. These findings might provide a novel insight into HBV-induced HCC metastasis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

HBx and HBs regulate RhoC expression by upregulating transcription factor Ets-1

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1655-1
Publisher site
See Article on Publisher Site

Abstract

The available evidence suggests that HBV proteins play an important role in the development of hepatocellular carcinoma (HCC). RhoC, a member of the Rho subfamily of the Ras superfamily of homologous genes, had been implicated in tumorigenesis and tumor progression. In a previous study, we demonstrated that HBx and HBs could up-regulate RhoC expression by enhancing its promoter activity. However, the specific mechanisms remain unclear. Here, we demonstrate that overexpression of Ets-1 results in upregulation of RhoC promoter activity and mRNA and protein levels. Expression of transcription factor Ets-1 was significantly higher in HepG2.2.15 cells than that in HepG2 cells. Meanwhile, infection of HepG2 cells with an HBV-adenovirus recombinant virus led to up-regulation of Ets-1. Of the four HBV proteins, HBx and HBs, could increase expression of Ets-1, which consequently contributed to the upregulation of RhoC. These findings might provide a novel insight into HBV-induced HCC metastasis.

Journal

Archives of VirologySpringer Journals

Published: Aug 1, 2013

References

  • Hepatocellular carcinoma: epidemiology and molecular carcinogenesis
    El-Serag, HB; Rudolph, KL
  • ETS1 promotes chemoresistance and invasion of paclitaxel-resistant, hormone-refractory PC3 prostate cancer cells by up-regulating MDR1 and MMP9 expression
    Kato, T; Fujita, Y; Nakane, K; Kojima, T; Nozawa, Y; Deguchi, T; Ito, M
  • Ets-1 transcription is required in tissue factor driven microvessel formation and stabilization
    Gemma, A; Esther, P; Rosa, A; Sonia, E; Lina, B
  • Overexpression of Ets-1 proto-oncogene in latent and clinical prostatic carcinomas
    Alipov, G; Nakayama, T; Ito, M; Kawai, K; Naito, S; Nakashima, M; Niino, D; Sekine
  • Clinical significance of matrix metalloproteinase-7 and Ets-1 gene expression in patients with lung cancer
    Sasaki, H; Yukiue, H; Moiriyama, S; Kobayashi, Y; Nakashima, Y; Kaji, M; Kiriyama, M; Fukai, I; Yamakawa, Y; Fujii, Y

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