Hairy-root cultures of Achyranthes aspera Linn. as a novel route for the production of 20-hydroxyecdysone

Hairy-root cultures of Achyranthes aspera Linn. as a novel route for the production of... An efficient transformation system for hairy-root induction in Achyranthes aspera L. was established and investigated as a novel method for the production of 20-hydroxyecdysone. Four Agrobacterium rhizogenes strains (MTCC 2364, NCIM 5140, A4, and ATCC 15834) were examined for their frequency of transformation in the presence of various concentrations of acetosyringone. Strain ATCC 15834 showed significantly higher transformation efficiency and was capable of inducing hairy roots from different explants (young leaf, hypocotyls, cotyledon, and stem segments) of A. aspera in the presence of 100 µM acetosyringone. The hairy root transformation of the lines (AS001 and AS002) was confirmed by PCR amplification of rol B locus using primers specific for rol B gene. Hairy roots grown in the Murashige and Skoog (MS) liquid medium augmented with 30 g/L sucrose exhibited the highest biomass accumulation and this medium was found to be superior to Gamborg’s B5 medium and Whites root culture medium. HPLC and LC-Q-TOF analyses of the hairy-root extract of A. aspera revealed the ability of hairy-root lines to synthesise the phytoecdysteroid 20-hydroxyecdysone. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Physiologiae Plantarum Springer Journals

Hairy-root cultures of Achyranthes aspera Linn. as a novel route for the production of 20-hydroxyecdysone

Loading next page...
 
/lp/springer_journal/hairy-root-cultures-of-achyranthes-aspera-linn-as-a-novel-route-for-6XYB672D35
Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków
Subject
Life Sciences; Plant Physiology; Plant Genetics and Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture
ISSN
0137-5881
eISSN
1861-1664
D.O.I.
10.1007/s11738-017-2555-x
Publisher site
See Article on Publisher Site

Abstract

An efficient transformation system for hairy-root induction in Achyranthes aspera L. was established and investigated as a novel method for the production of 20-hydroxyecdysone. Four Agrobacterium rhizogenes strains (MTCC 2364, NCIM 5140, A4, and ATCC 15834) were examined for their frequency of transformation in the presence of various concentrations of acetosyringone. Strain ATCC 15834 showed significantly higher transformation efficiency and was capable of inducing hairy roots from different explants (young leaf, hypocotyls, cotyledon, and stem segments) of A. aspera in the presence of 100 µM acetosyringone. The hairy root transformation of the lines (AS001 and AS002) was confirmed by PCR amplification of rol B locus using primers specific for rol B gene. Hairy roots grown in the Murashige and Skoog (MS) liquid medium augmented with 30 g/L sucrose exhibited the highest biomass accumulation and this medium was found to be superior to Gamborg’s B5 medium and Whites root culture medium. HPLC and LC-Q-TOF analyses of the hairy-root extract of A. aspera revealed the ability of hairy-root lines to synthesise the phytoecdysteroid 20-hydroxyecdysone.

Journal

Acta Physiologiae PlantarumSpringer Journals

Published: Oct 22, 2017

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off