Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris

Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the... Biotechnol Lett (2018) 40:1149–1156 https://doi.org/10.1007/s10529-018-2571-y OR IGINAL RESEARCH PAPER Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris . . . Olufemi Emmanuel Bankefa Meiyu Wang Taicheng Zhu Yin Li Received: 31 March 2018 / Accepted: 18 May 2018 / Published online: 21 May 2018 Springer Science+Business Media B.V., part of Springer Nature 2018 Abstract b-mannanase production from 73 to 108.5 U b- -1 Objectives To systematically explore the effects of mannanase mL while PpHac1p had no impact in overexpressing Hac1p homologues from different shake flask culture. Moreover, HsXbp1 led to 41 and sources on protein secretion in Pichia pastoris system. 67% increases in b-mannanase production in the Results Effects of Hac1p homologues encompassing single- and four-copy strain, respectively in 1-L P. pastoris (PpHac1p), S. cerevisiae (ScHac1p), laboratory fermenter. Transcription analysis of indica- Trichoderma reesei (TrHac1p) and Homo sapiens tive chaperones suggested that HsXbp1 may cause a (HsXbp1), on secretion of three reporter proteins—b- stronger and prolonged activation of the UPR target galactosidase, b-mannanase and glucose oxidase were chaperone genes. investigated. No individual Hac1p was optimal for all Conclusion Mammalian HsXbp1 worked better than the enzymes. Rather, by testing a set http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Letters Springer Journals

Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris

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Publisher
Springer Netherlands
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Life Sciences; Microbiology; Biotechnology; Applied Microbiology; Biochemistry, general
ISSN
0141-5492
eISSN
1573-6776
D.O.I.
10.1007/s10529-018-2571-y
Publisher site
See Article on Publisher Site

Abstract

Biotechnol Lett (2018) 40:1149–1156 https://doi.org/10.1007/s10529-018-2571-y OR IGINAL RESEARCH PAPER Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris . . . Olufemi Emmanuel Bankefa Meiyu Wang Taicheng Zhu Yin Li Received: 31 March 2018 / Accepted: 18 May 2018 / Published online: 21 May 2018 Springer Science+Business Media B.V., part of Springer Nature 2018 Abstract b-mannanase production from 73 to 108.5 U b- -1 Objectives To systematically explore the effects of mannanase mL while PpHac1p had no impact in overexpressing Hac1p homologues from different shake flask culture. Moreover, HsXbp1 led to 41 and sources on protein secretion in Pichia pastoris system. 67% increases in b-mannanase production in the Results Effects of Hac1p homologues encompassing single- and four-copy strain, respectively in 1-L P. pastoris (PpHac1p), S. cerevisiae (ScHac1p), laboratory fermenter. Transcription analysis of indica- Trichoderma reesei (TrHac1p) and Homo sapiens tive chaperones suggested that HsXbp1 may cause a (HsXbp1), on secretion of three reporter proteins—b- stronger and prolonged activation of the UPR target galactosidase, b-mannanase and glucose oxidase were chaperone genes. investigated. No individual Hac1p was optimal for all Conclusion Mammalian HsXbp1 worked better than the enzymes. Rather, by testing a set

Journal

Biotechnology LettersSpringer Journals

Published: May 21, 2018

References

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