Biotechnol Lett (2018) 40:1149–1156 https://doi.org/10.1007/s10529-018-2571-y OR IGINAL RESEARCH PAPER Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris . . . Olufemi Emmanuel Bankefa Meiyu Wang Taicheng Zhu Yin Li Received: 31 March 2018 / Accepted: 18 May 2018 / Published online: 21 May 2018 Springer Science+Business Media B.V., part of Springer Nature 2018 Abstract b-mannanase production from 73 to 108.5 U b- -1 Objectives To systematically explore the effects of mannanase mL while PpHac1p had no impact in overexpressing Hac1p homologues from different shake ﬂask culture. Moreover, HsXbp1 led to 41 and sources on protein secretion in Pichia pastoris system. 67% increases in b-mannanase production in the Results Effects of Hac1p homologues encompassing single- and four-copy strain, respectively in 1-L P. pastoris (PpHac1p), S. cerevisiae (ScHac1p), laboratory fermenter. Transcription analysis of indica- Trichoderma reesei (TrHac1p) and Homo sapiens tive chaperones suggested that HsXbp1 may cause a (HsXbp1), on secretion of three reporter proteins—b- stronger and prolonged activation of the UPR target galactosidase, b-mannanase and glucose oxidase were chaperone genes. investigated. No individual Hac1p was optimal for all Conclusion Mammalian HsXbp1 worked better than the enzymes. Rather, by testing a set
Biotechnology Letters – Springer Journals
Published: May 21, 2018
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