Griffithsin inhibits Japanese encephalitis virus infection in vitro and in vivo

Griffithsin inhibits Japanese encephalitis virus infection in vitro and in vivo Griffithsin (GRFT) is a broad-spectrum antiviral protein that is effective against several glycosylated viruses. Here, we have evaluated the in vitro and in vivo antiviral activities of GRFT against Japanese encephalitis virus (JEV) infection. In vitro experiments showed that treatment of JEV with GRFT before inoculation of BHK-21 cells inhibited infection in a dose-dependent manner, with 99 % inhibition at 100 μg/ml and a 50 % inhibitory concentration ( IC 50 ) of 265 ng/ml (20 nM). Binding assays suggested that binding of GRFT to JEV virions inhibited JEV infection. In vivo experiment showed that GRFT (5 mg/kg) administered intraperitoneally before virus infection could completely prevent mortality in mice challenged intraperitoneally with a lethal dose of JEV. Our study also suggested that GRFT prevents JEV infection at the entry phase by targeting the virus. Collectively, our data demonstrate that GRFT is an antiviral agent with potential application in the development of therapeutics against JEV or other flavivirus infections. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Griffithsin inhibits Japanese encephalitis virus infection in vitro and in vivo

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-012-1489-2
Publisher site
See Article on Publisher Site

Abstract

Griffithsin (GRFT) is a broad-spectrum antiviral protein that is effective against several glycosylated viruses. Here, we have evaluated the in vitro and in vivo antiviral activities of GRFT against Japanese encephalitis virus (JEV) infection. In vitro experiments showed that treatment of JEV with GRFT before inoculation of BHK-21 cells inhibited infection in a dose-dependent manner, with 99 % inhibition at 100 μg/ml and a 50 % inhibitory concentration ( IC 50 ) of 265 ng/ml (20 nM). Binding assays suggested that binding of GRFT to JEV virions inhibited JEV infection. In vivo experiment showed that GRFT (5 mg/kg) administered intraperitoneally before virus infection could completely prevent mortality in mice challenged intraperitoneally with a lethal dose of JEV. Our study also suggested that GRFT prevents JEV infection at the entry phase by targeting the virus. Collectively, our data demonstrate that GRFT is an antiviral agent with potential application in the development of therapeutics against JEV or other flavivirus infections.

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2013

References

  • Structure of a flavivirus envelope glycoprotein in its low-pH-induced membrane fusion conformation
    Bressanelli, S; Stiasny, K; Allison, SL; Stura, EA; Duquerroy, S; Lescar, J; Heinz, FX; Rey, FA
  • Inhibition of Japanese encephalitis virus infection by the sulfated polysaccharides extracts from Ulva lactuca
    Chiu, YH; Chan, Y-L; Li, T-L; Wu, CJ
  • Griffithsin, a potent HIV entry inhibitor, is an excellent candidate for anti-HIV microbicide
    Emau, P; Tian, B; O’keefe, B; Mori, T; McMahon, J; Palmer, K; Jiang, Y; Bekele, G; Tsai, C
  • Recombinant production of anti-HIV protein, griffithsin, by auto-induction in a fermentor culture
    Giomarelli, B; Schumacher, KM; Taylor, TE; Sowder, RC; Hartley, JL; McMahon, JB; Mori, T

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