Green tea polyphenols in combination with copper(II) induce apoptosis in HeLa cells

Green tea polyphenols in combination with copper(II) induce apoptosis in HeLa cells In the present investigation we report that the natural antioxidant green tea polyphenols (GTPs) could induce apoptosis in HeLa cells in the presence of copper cation. After treatment with low concentrations of GTP (5-15 μM) plus 2 μM Cu2+, HeLa cells died gradually as measured by the MTT assay. Agarose gel electrophoresis analysis of DNA extracted from HeLa cells exposed to GTP/Cu2+ showed typical internucleosomal DNA fragmentation (DNA ladder), which was also confirmed by DNA content analysis by flow cytometry. Morphological observation of HeLa cells treated with GTP/Cu2+ by transmission electronic microscopy also showed typical apoptotic features including cell shrinkage, chromatin condensation, and the formation of apoptotic bodies. Laser confocal microscopy determination of intracellular calcium level revealed that exposure to GTP/Cu2+ triggered increase of cytosolic calcium ion, which may be involved in the process of apoptosis. This evidence suggested that GTPs might be used as potential therapeutic agent for the treatment of cancer. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Green tea polyphenols in combination with copper(II) induce apoptosis in HeLa cells

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Publisher
Brill Academic Publishers
Copyright
Copyright © 2002 by VSP 2002
Subject
Chemistry; Inorganic Chemistry; Physical Chemistry; Catalysis
ISSN
0922-6168
eISSN
1568-5675
D.O.I.
10.1163/15685670260373344
Publisher site
See Article on Publisher Site

Abstract

In the present investigation we report that the natural antioxidant green tea polyphenols (GTPs) could induce apoptosis in HeLa cells in the presence of copper cation. After treatment with low concentrations of GTP (5-15 μM) plus 2 μM Cu2+, HeLa cells died gradually as measured by the MTT assay. Agarose gel electrophoresis analysis of DNA extracted from HeLa cells exposed to GTP/Cu2+ showed typical internucleosomal DNA fragmentation (DNA ladder), which was also confirmed by DNA content analysis by flow cytometry. Morphological observation of HeLa cells treated with GTP/Cu2+ by transmission electronic microscopy also showed typical apoptotic features including cell shrinkage, chromatin condensation, and the formation of apoptotic bodies. Laser confocal microscopy determination of intracellular calcium level revealed that exposure to GTP/Cu2+ triggered increase of cytosolic calcium ion, which may be involved in the process of apoptosis. This evidence suggested that GTPs might be used as potential therapeutic agent for the treatment of cancer.

Journal

Research on Chemical IntermediatesSpringer Journals

Published: Oct 13, 2004

References

  • Studies on protective mechanisms of four components of green tea polyphenols against lipid peroxidation in synaptosomes
    Guo, Q.; Zhao, B. L.; Li, M. F.; Shen, S. R.; Xin, W. J.
  • Screening of anticarcinogenic ingredients in tea polyphenols
    Han, C.
  • Preventive effect of Ginkgo biloba extract on apoptosis in rat cerebellar neuronal cells induced by hydroxyl radicals
    Ni, Y. C.; Zhao, B. L.; Hou, J. W.; Xin, W. J.

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