Grande retrotransposons contain an accessory gene in the unusually long 3′-internal region that encodes a nuclear protein transcribed from its own promoter

Grande retrotransposons contain an accessory gene in the unusually long 3′-internal region that... LTR retrotransposons are major components of plant genomes playing important roles in the evolution of their host genomes, for example, generating new genes or providing new promoters to existing genes. The Grande family of retrotransposons is present in Zea species and is characterized by an unusually long internal region due to the presence of a 7-kbp region between the gag-pol coding region and the 3′LTR. We demonstrate here that such unusual sequence is present in the great majority of Grande copies in maize genome. This region contains a gene, gene23, which is transcribed from its own promoter in antisense orientation to the gag-pol genes. The expression of gene23 is ubiquitous, and its promoter contains all the putative consensus sequences typical of eukaryotic promoters, being able to direct GUS expression in different plant species and organs. The coding region of gene23 is conserved in most Grande copies and encodes a protein rich in glycine, serine, and acidic amino acids that shows no significant similarity with any protein of known function. Nevertheless, the C- and N-terminal parts are rich in basic amino acids, and these are interspersed with other amino acids in its C-terminus, compatible with a putative DNA-binding function. It contains a nuclear localization signal KRKR motif in the N-terminus. Fusions to GFP demonstrate that this protein localizes in the nucleus. We discuss the possible origin of gene23 and the potential function of its encoded protein. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Grande retrotransposons contain an accessory gene in the unusually long 3′-internal region that encodes a nuclear protein transcribed from its own promoter

Loading next page...
 
/lp/springer_journal/grande-retrotransposons-contain-an-accessory-gene-in-the-unusually-JgKa3yYOT1
Publisher
Springer Netherlands
Copyright
Copyright © 2013 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-013-0019-2
Publisher site
See Article on Publisher Site

Abstract

LTR retrotransposons are major components of plant genomes playing important roles in the evolution of their host genomes, for example, generating new genes or providing new promoters to existing genes. The Grande family of retrotransposons is present in Zea species and is characterized by an unusually long internal region due to the presence of a 7-kbp region between the gag-pol coding region and the 3′LTR. We demonstrate here that such unusual sequence is present in the great majority of Grande copies in maize genome. This region contains a gene, gene23, which is transcribed from its own promoter in antisense orientation to the gag-pol genes. The expression of gene23 is ubiquitous, and its promoter contains all the putative consensus sequences typical of eukaryotic promoters, being able to direct GUS expression in different plant species and organs. The coding region of gene23 is conserved in most Grande copies and encodes a protein rich in glycine, serine, and acidic amino acids that shows no significant similarity with any protein of known function. Nevertheless, the C- and N-terminal parts are rich in basic amino acids, and these are interspersed with other amino acids in its C-terminus, compatible with a putative DNA-binding function. It contains a nuclear localization signal KRKR motif in the N-terminus. Fusions to GFP demonstrate that this protein localizes in the nucleus. We discuss the possible origin of gene23 and the potential function of its encoded protein.

Journal

Plant Molecular BiologySpringer Journals

Published: Feb 20, 2013

References

  • Chromosome localization and characterization of a family of long repetitive DNA elements from the genus Zea
    Aledo, R; Raz, R; Monfort, A; Vicient, CM; Puigdomenech, P; Martinez-Izquierdo, JA
  • Host factors that affect Ty3 retrotransposition in Saccharomyces cerevisiae
    Aye, M; Irwin, B; Beliakova-Bethell, N; Chen, E; Garrus, J; Sandmeyer, S
  • Transposable elements, gene creation and genome rearrangement in flowering plants
    Bennetzen, JL
  • Cellular oncogenes and retroviruses
    Bishop, JM
  • Nuclear localization signals (NLS)
    Boulikas, T
  • Lentiviral nuclear import: a complex interplay between virus and host
    Rijck, J; Vandekerckhove, L; Christ, F; Debyser, Z
  • Bs1, a new chimeric gene formed by retrotransposon-mediated exon shuffling in maize
    Elrouby, N; Bureau, TE
  • HIV-1: fifteen proteins and an RNA
    Frankel, AD; Young, JA
  • Plant chromosomal high mobility group (HMG) proteins
    Grasser, KD
  • Speciation and domestication in maize and its wild relatives: evidence from the globulin-1 gene
    Hilton, H; Gaut, BS

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off