Access the full text.
Sign up today, get DeepDyve free for 14 days.
Han Ding, Chunsu Liang, Kangbo Sun, Hui Wang, J. Hiltunen, Zhijun Chen, Jia-cong Shen (2014)
Dithiothreitol-capped fluorescent gold nanoclusters: an efficient probe for detection of copper(II) ions in aqueous solution.Biosensors & bioelectronics, 59
Yanyan Cui, Yaling Wang, Ru Liu, Zhipeng Sun, Yueteng Wei, Yuliang Zhao, Xueyun Gao (2011)
Serial silver clusters biomineralized by one peptide.ACS nano, 5 11
Zonghua Wang, Na Sun, Yao He, Yang Liu, Jinghong Li (2014)
DNA assembled gold nanoparticles polymeric network blocks modular highly sensitive electrochemical biosensors for protein kinase activity analysis and inhibition.Analytical chemistry, 86 12
R. Agnes, F. Jernigan, Jennifer Shell, Vyas Sharma, D. Lawrence (2010)
Suborganelle sensing of mitochondrial cAMP-dependent protein kinase activity.Journal of the American Chemical Society, 132 17
Chung-Liang Wang, Lan-Yi Wei, C. Yuan, K. Hwang (2012)
Reusable amperometric biosensor for measuring protein tyrosine kinase activity.Analytical chemistry, 84 2
Xue Hu, Xiuming Wu, Xin-Xin Fang, Zaijun Li, Guangli Wang (2016)
Switchable fluorescence of gold nanoclusters for probing the activity of alkaline phosphatase and its application in immunoassay.Biosensors & bioelectronics, 77
Jie Bai, Yunjie Zhao, Zhibin Wang, Chenghui Liu, Yu-cong Wang, Zhengping Li (2013)
Dual-readout fluorescent assay of protein kinase activity by use of TiO2-coated magnetic microspheres.Analytical chemistry, 85 9
Jiang Zhou, Xiahong Xu, Xin Liu, Hao Li, Z. Nie, Meng Qing, Y. Huang, S. Yao (2014)
A gold nanoparticles colorimetric assay for label-free detection of protein kinase activity based on phosphorylation protection against exopeptidase cleavage.Biosensors & bioelectronics, 53
Jiang Zhou, Xiahong Xu, Wei Liu, Xin Liu, Z. Nie, Meng Qing, L. Nie, S. Yao (2013)
Graphene oxide-peptide nanocomplex as a versatile fluorescence probe of protein kinase activity based on phosphorylation protection against carboxypeptidase digestion.Analytical chemistry, 85 12
Guoyan Lu, Penglong Tan, Chunyang Lei, Z. Nie, Y. Huang, Shouzhou Yao (2014)
Fluorescent detection of protein kinase based on positively charged gold nanoparticles.Talanta, 128
J. Qiao, Xiaoyu Mu, L. Qi, Jingjing Deng, L. Mao (2013)
Folic acid-functionalized fluorescent gold nanoclusters with polymers as linkers for cancer cell imaging.Chemical communications, 49 73
Shoujiang Xu, Yang Liu, Taihong Wang, Jinghong Li (2010)
Highly sensitive electrogenerated chemiluminescence biosensor in profiling protein kinase activity and inhibition using gold nanoparticle as signal transduction probes.Analytical chemistry, 82 22
Mehri Ghazaghi, H. Mousavi, H. Shirkhanloo, A. Rashidi (2016)
Ultrasound assisted dispersive micro solid-phase extraction of four tyrosine kinase inhibitors from serum and cerebrospinal fluid by using magnetic nanoparticles coated with nickel-doped silica as an adsorbentMicrochimica Acta, 183
Congcong Shen, Xiaodong Xia, Shengqiang Hu, Minghui Yang, Jianxiu Wang (2015)
Silver nanoclusters-based fluorescence assay of protein kinase activity and inhibition.Analytical chemistry, 87 1
Qunzhao Wang, S. Cahill, M. Blumenstein, D. Lawrence (2006)
Self-reporting fluorescent substrates of protein tyrosine kinases.Journal of the American Chemical Society, 128 6
Pu Zhang, Xiao Yang, Yi Wang, N. Zhao, Zuhong Xiong, C. Huang (2014)
Rapid synthesis of highly luminescent and stable Au20 nanoclusters for active tumor-targeted imaging in vitro and in vivo.Nanoscale, 6 4
M. Schäferling, O. Wolfbeis (2007)
Europium tetracycline as a luminescent probe for nucleoside phosphates and its application to the determination of kinase activity.Chemistry, 13 15
Congcong Shen, Kaina Zhang, Nanxing Gao, Shi Wei, Guimei Liu, Yuanlin Chai, Minghui Yang (2016)
Colorimetric and electrochemical determination of the activity of protein kinase based on retarded particle growth due to binding of phosphorylated peptides to DNA – capped silver nanoclustersMicrochimica Acta, 183
W. Song, Ruping Liang, Ying Wang, Li Zhang, Jianding Qiu (2016)
Gold nanoclusters-based dual-emission ratiometric fluorescence probe for monitoring protein kinaseSensors and Actuators B-chemical, 226
J. Schlessinger (2000)
Cell Signaling by Receptor Tyrosine KinasesCell, 103
Y. Qiao, Yan Zhang, Caihong Zhang, Lihong Shi, Guo-mei Zhang, S. Shuang, C. Dong, Huimin Ma (2016)
Water-soluble gold nanoclusters-based fluorescence probe for highly selective and sensitive detection of Hg2+Sensors and Actuators B-chemical, 224
S. Ding, Chunmei Li, Bu-hong Gao, O. Kargbo, N. Wan, Xi Chen, Chang-mei Zhou (2014)
Probing phosphate ion via the europium(III)-modulated fluorescence of gold nanoclustersMicrochimica Acta, 181
B. Houseman, Joon Huh, S. Kron, M. Mrksich (2002)
Peptide chips for the quantitative evaluation of protein kinase activityNature Biotechnology, 20
Deborah Rothman, Melissa Shults, B. Imperiali (2005)
Chemical approaches for investigating phosphorylation in signal transduction networks.Trends in cell biology, 15 9
W. Song, Ruping Liang, Ying Wang, Li Zhang, Jianding Qiu (2015)
Green synthesis of peptide-templated gold nanoclusters as novel fluorescence probes for detecting protein kinase activity.Chemical communications, 51 49
G. Manning, D. Whyte, R. Martinez, T. Hunter, S. Sudarsanam (2002)
The Protein Kinase Complement of the Human GenomeScience, 298
S. Ding, Chunmei Li, Ning Bao (2015)
Off-on phosphorescence assay of heparin via gold nanoclusters modulated with protamine.Biosensors & bioelectronics, 64
Xiahong Xu, Xin Liu, Z. Nie, Yuliang Pan, Manli Guo, S. Yao (2011)
Label-free fluorescent detection of protein kinase activity based on the aggregation behavior of unmodified quantum dots.Analytical chemistry, 83 1
Li Shang, S. Dong, G. Nienhaus (2011)
Ultra-small fluorescent metal nanoclusters: Synthesis and biological applicationsNano Today, 6
Lei Wang, Xu Yan, X. Su (2016)
A label-free and sensitive fluorescent assay for one step detection of protein kinase activity and inhibition.Analytica chimica acta, 935
Libing Zhang, E. Wang (2014)
Metal nanoclusters: New fluorescent probes for sensors and bioimagingNano Today, 9
Lianzhe Hu, Shuang Han, S. Parveen, Yali Yuan, Ling Zhang, Guobao Xu (2012)
Highly sensitive fluorescent detection of trypsin based on BSA-stabilized gold nanoclusters.Biosensors & bioelectronics, 32 1
Lianzhe Hu, Lin Deng, S. Alsaiari, Dingyuan Zhang, N. Khashab (2014)
"Light-on" sensing of antioxidants using gold nanoclusters.Analytical chemistry, 86 10
Jie Bai, Chenghui Liu, Te-min Yang, Fangfang Wang, Zhengping Li (2013)
A versatile platform for highly sensitive detection of kinase activity based on metal ion-mediated FRET using an anionic conjugated polymer.Chemical communications, 49 37
Ying Wang, Li Zhang, Ruping Liang, Jianliang Bai, Jianding Qiu (2013)
Using graphene quantum dots as photoluminescent probes for protein kinase sensing.Analytical chemistry, 85 19
W. Song, Ying Wang, Ruping Liang, Li Zhang, Jianding Qiu (2015)
Label-free fluorescence assay for protein kinase based on peptide biomineralized gold nanoclusters as signal sensing probe.Biosensors & bioelectronics, 64
A sensitive fluorometric assay is reported for label-free detection of the activity of protein kinase (PKA) and of its inhibitors. It is based on the europium(III)-modulated fluorescence of peptide-stabilized gold nanoclusters (peptide-AuNCs). Both adenosine-5′-triphosphate (ATP) and adenosine-5′-diphosphate (ADP) (formed from ATP by PKA-catalyzed hydrolysis) enhance the fluorescence of peptide-AuNCs with its excitation/emission peaks of 330/405 nm. The addition of Eu(III) quenches the fluorescence of the ATP/peptide-AuNC system to the original fluorescence level of peptide-AuNCs, while addition of Eu(III) has no effect on the fluorescence of the ATP/PKA/peptide-AuNC system. Based on this “turn-on” method, the activity of PKA can be detected with high sensitivity in 0.05–1.6 U⋅mL−1 activity range, with a detection limit of 0.02 U⋅mL−1 (3σ). The feasibility of this method for screening of inhibitors was also studied. The IC50 value for the commercially available inhibitor H-89 was found to be 43 nM. Moreover, the detection scheme was also applied to monitoring the drug-stimulated activation of PKA in HepG-2 cell lysates.
Microchimica Acta – Springer Journals
Published: Jun 14, 2017
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.