In this communication, a gold-coated polydimethylsiloxane (PDMS) chip with cell-sized microwells was prepared through a stamping and spraying process that was applied directly for high-throughput electrochemiluminescence (ECL) analysis of intracellular glucose at single cells. As compared with the previous multiple-step fabrication of photoresist-based microwells on the electrode, the preparation process is simple and offers fresh electrode surface for higher luminescence intensity. More luminescence intensity was recorded from cell-retained microwells than that at the planar region among the microwells that was correlated with the content of intracellular glucose. The successful monitoring of intracellular glucose at single cells using this PDMS chip will provide an alternative strategy for high-throughput single-cell analysis. . . . . Keywords Single cell analysis PDMS microwells Electrochemiluminescence imaging Intracellular glucose Cellular heterogeneity Introduction and chemical analyses , have been widely developed for single-cell study to provide valuable information about chem- Recent evidence from single-cell analysis reveals significant ical composition and local activity of cells. cellular heterogeneity in the same type of cells within organ- In the past 5 years, our group developed an isms [1, 2]. The full understanding of cellular signaling at electrochemiluminescence (ECL) assay for the detection of single-cell level will greatly promote the
Analytical and Bioanalytical Chemistry – Springer Journals
Published: Jun 4, 2018
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