Ghrelin knockout mice display defective skeletal muscle regeneration and impaired satellite cell self-renewal

Ghrelin knockout mice display defective skeletal muscle regeneration and impaired satellite cell... Purpose Muscle regeneration depends on satellite cells (SCs), quiescent precursors that, in consequence of injury or pathological states such as muscular dystrophies, activate, proliferate, and differentiate to repair the damaged tissue. A subset of SCs undergoes self-renewal, thus preserving the SC pool and its regenerative potential. The peptides produced by the ghrelin gene, i.e., acylated ghrelin (AG), unacylated ghrelin (UnAG), and obestatin (Ob), affect skeletal muscle biology in several ways, not always with overlapping effects. In particular, UnAG and Ob promote SC self-renewal and myoblast differentiation, thus fostering muscle regeneration. Methods To delineate the endogenous contribution of preproghrelin in muscle regeneration, we evaluated the repair process −/− in Ghrl mice upon CTX-induced injury. −/− Results Although muscles from Ghrl mice do not visibly differ from WT muscles in term of weight, structure, and SCs −/− content, muscle regeneration after CTX-induced injury is impaired in Ghrl mice, indicating that ghrelin-derived peptides actively participate in muscle repair. Remarkably, the lack of ghrelin gene impacts SC self-renewal during regeneration. Conclusions Although we cannot discern the specific Ghrl-derived peptide responsible for such activities, these data indicate that Ghrl contributes to a proper muscle regeneration. ● ● ● Keywords Ghrelin knockout Skeletal muscle regeneration http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Endocrine Springer Journals

Ghrelin knockout mice display defective skeletal muscle regeneration and impaired satellite cell self-renewal

Download PDF
7 pages
Read Article

Loading next page...
 
/lp/springer_journal/ghrelin-knockout-mice-display-defective-skeletal-muscle-regeneration-QjqnWP5Cb1
Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Medicine & Public Health; Endocrinology; Diabetes; Internal Medicine; Science, Humanities and Social Sciences, multidisciplinary
ISSN
1355-008X
eISSN
1559-0100
DOI
10.1007/s12020-018-1606-4
Publisher site
See Article on Publisher Site

Abstract

Purpose Muscle regeneration depends on satellite cells (SCs), quiescent precursors that, in consequence of injury or pathological states such as muscular dystrophies, activate, proliferate, and differentiate to repair the damaged tissue. A subset of SCs undergoes self-renewal, thus preserving the SC pool and its regenerative potential. The peptides produced by the ghrelin gene, i.e., acylated ghrelin (AG), unacylated ghrelin (UnAG), and obestatin (Ob), affect skeletal muscle biology in several ways, not always with overlapping effects. In particular, UnAG and Ob promote SC self-renewal and myoblast differentiation, thus fostering muscle regeneration. Methods To delineate the endogenous contribution of preproghrelin in muscle regeneration, we evaluated the repair process −/− in Ghrl mice upon CTX-induced injury. −/− Results Although muscles from Ghrl mice do not visibly differ from WT muscles in term of weight, structure, and SCs −/− content, muscle regeneration after CTX-induced injury is impaired in Ghrl mice, indicating that ghrelin-derived peptides actively participate in muscle repair. Remarkably, the lack of ghrelin gene impacts SC self-renewal during regeneration. Conclusions Although we cannot discern the specific Ghrl-derived peptide responsible for such activities, these data indicate that Ghrl contributes to a proper muscle regeneration. ● ● ● Keywords Ghrelin knockout Skeletal muscle regeneration

Journal

Endocrine – Springer Journals

Published: May 30, 2018

References

  • Pax7 is required for the specification of myogenic satellite cells
    Seale, P; Sabourin, LA; Girgis-Gabardo, A; Mansouri, A; Gruss, P; Rudnicki, MA
  • The obestatin/GPR39 system is up-regulated by muscle injury and functions as an autocrine regenerative system
    Gurriarán-Rodríguez, U; Santos-zas, I; Al-massadi, O; Mosteiro, CS; Nogueiras, R; Crujeiras, AB; Seoane, LM; Señarís, J; Gallego, R; Felipe, F; Pazos, Y; Camiña, JP
  • Unacylated ghrelin enhances satellite cell function and relieves the dystrophic phenotype in Duchenne muscular dystrophy mdx model
    Reano, S; Angelino, E; Ferrara, M; Malacarne, V; Sustova, H; Sabry, O; Agosti, E; Clerici, S; Ruozi, G; Zentilin, L; Prodam, F; Geuna, S; Giacca, M; Graziani, A; Filigheddu, N
  • Action of obestatin in skeletal muscle repair: stem cell expansion, muscle growth, and microenvironment remodeling
    Gurriarán-Rodríguez, U; Santos-zas, I; González-sánchez, J; Beiroa, D; Moresi, V; Mosteiro, CS; Lin, W; Viñuela, JE; Señarís, J; García-caballero, T; Casanueva, FF; Nogueiras, R; Gallego, R; Renaud, J; Adamo, S; Pazos, Y; Camiña, JP
  • AAV-mediated in vivo functional selection of tissue-protective factors against ischaemia
    Ruozi, G; Bortolotti, F; Falcione, A; Ferro, MD; Ukovich, L; Macedo, A; Zentilin, L; Filigheddu, N; Cappellari, GG; Baldini, G; Zweyer, M; Barazzoni, R; Graziani, A; Zacchigna, S; Giacca, M
  • Unacylated ghrelin promotes skeletal muscle regeneration following hindlimb ischemia via SOD-2-mediated miR-221/222 expression
    Togliatto, GG; Trombetta, A; Dentelli, P; Cotogni, P; Rosso, A; Matthias, H; Granata, R; Ghigo, E; Brizzi, MF; Togliatto, G; Trombetta, A; Dentelli, P; Cotogni, P; Rosso, A
  • Ghrelin and des-acyl ghrelin promote differentiation and fusion of C2C12 skeletal muscle cells
    Filigheddu, N; Gnocchi, VF; Coscia, M; Cappelli, M; Porporato, PE; Taulli, R; Traini, S; Baldanzi, G; Chianale, F; Cutrupi, S; Arnoletti, E; Ghe, C; Fubini, A; Surico, N; Sinigaglia, F; Ponzetto, C; Muccioli, G; Crepaldi, T; Graziani, A
  • β-Arrestin scaffolds and signaling elements essential for the obestatin/GPR39 system that determine the myogenic program in human myoblast cells
    Santos-Zas, I; Gurriarán-Rodríguez, U; Cid-Díaz, T; Figueroa, G; González-Sánchez, J; Bouzo-Lorenzo, M; Mosteiro, CS; Señarís, J; Casanueva, FF; Casabiell, X; Gallego, R; Pazos, Y; Mouly, V; Camiña, JP
  • Acylated and unacylated ghrelin inhibit doxorubicin-induced apoptosis in skeletal muscle
    Yu, AP; Pei, XM; Sin, TK; Yip, SP; Yung, BY; Chan, LW; Wong, CS; Siu, PM
  • Ghrelin inhibits skeletal muscle protein breakdown in rats with thermal injury through normalizing elevated expression of E3 ubiquitin ligases MuRF1 and MAFbx
    Balasubramaniam, A; Joshi, R; Su, C; Friend, LA; Sheriff, S; Kagan, RJ; James, JH
  • Des-acyl ghrelin exhibits pro-anabolic and anti-catabolic effects on C2C12 myotubes exposed to cytokines and reduces burn-induced muscle proteolysis in rats
    Sheriff, S; Kadeer, N; Joshi, R; Ann, L; James, JH; Balasubramaniam, A
  • Acylated and unacylated ghrelin impair skeletal muscle atrophy in mice
    Porporato, PE; Filigheddu, N; Reano, S; Ferrara, M; Angelino, E; Gnocchi, VF; Prodam, F; Ronchi, G; Fagoonee, S; Fornaro, M; Chianale, F; Baldanzi, G; Surico, N; Sinigaglia, F; Perroteau, I; Smith, RG; Sun, Y; Geuna, S; Graziani, A
  • Improvement of physical decline through combined effects of muscle enhancement and mitochondrial activation by a gastric hormone ghrelin in male 5/6Nx CKD model mice
    Tamaki, M; Hagiwara, A; Miyashita, K; Wakino, S; Inoue, H; Fujii, K; Fujii, C; Sato, M; Mitsuishi, M; Muraki, A; Hayashi, K; Doi, T; Itoh, H
  • Unacylated ghrelin normalizes skeletal muscle oxidative stress and prevents muscle catabolism by enhancing tissue mitophagy in experimental chronic kidney disease
    Cappellari, GG; Semolic, A; Ruozi, G; Vinci, P; Guarnieri, G; Bortolotti, F; Barbetta, D; Zanetti, M; Giacca, M; Barazzoni, R
  • Acylated and unacylated ghrelin inhibit atrophy in myotubes co-cultured with colon carcinoma cells
    Zeng, X; Chen, S; Yang, Y; Ke, Z
  • Therapeutic potential of ghrelin treatment for unloading-induced muscle atrophy in mice
    Koshinaka, K; Toshinai, K; Mohammad, A; Noma, K; Oshikawa, M; Ueno, H; Yamaguchi, H; Nakazato, M
  • Ghrelin improves body weight loss and skeletal muscle catabolism associated with angiotensin II-induced cachexia in mice
    Sugiyama, M; Yamaki, A; Furuya, M; Inomata, N; Minamitake, Y; Ohsuye, K; Kangawa, K
  • Ghrelin relieves cancer cachexia associated with the development of lung adenocarcinoma in mice
    Tsubouchi, H; Yanagi, S; Miura, A; Matsumoto, N; Kangawa, K; Nakazato, M
  • Ghrelin prevents tumour- and cisplatin-induced muscle wasting: characterization of multiple mechanisms involved
    Chen, J; Splenser, A; Guillory, B; Luo, J; Mendiratta, M; Belinova, B; Halder, T; Zhang, G; Li, Y; Garcia, JM
  • Unacylated ghrelin reduces skeletal muscle reactive oxygen species generation and inflammation and prevents high-fat diet-induced hyperglycemia and whole-body insulin resistance in rodents
    Cappellari, GG; Zanetti, M; Semolic, A; Vinci, P; Ruozi, G; Falcione, A; Filigheddu, N; Guarnieri, G; Graziani, A; Giacca, M; Barazzoni, R
  • An early reduction in GH peak amplitude in preproghrelin-deficient male mice has a minor impact on linear growth
    Hassouna, R; Zizzari, P; Tomasetto, C; Veldhuis, JD; Fiquet, O; Labarthe, A; Cognet, J; Steyn, F; Chen, C; Epelbaum, J; Tolle, V
  • Sprouty1 regulates reversible quiescence of a self-renewing adult muscle stem cell pool during regeneration
    Shea, KL; Xiang, W; Laporta, VS; Licht, JD; Keller, C; Basson, MA; Brack, AS
  • Ghrelin gene products in acute and chronic inflammation
    Prodam, F; Filigheddu, N
  • Satellite cells and the muscle stem cell niche
    Yin, H; Price, F; Rudnicki, MA

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial