Genomic variability in Potato potyvirus Y (PVY): evidence that PVY N W and PVY NTN variants are single to multiple recombinants between PVY O and PVY N isolates

Genomic variability in Potato potyvirus Y (PVY): evidence that PVY N W and PVY NTN variants are... Fourteen Potato virus Y (PVY) isolates representative of PVY O , PVY N , PVY NTN and PVY N W groups were characterised at genomic level. Restriction fragment length polymorphism study (RFLP) of each gene of these isolates and sequencing of the first 2700 nucleotides of two PVY N W isolates were performed. A mosaic structure was revealed in PVY N W and PVY NTN genomes, which showed either PVY O or PVY N -like sequences, depending on the particular gene. Indeed, starting from the 5′-end, these isolates showed a switching, from PVY N - to PVY O -like sequence, in the HC-Pro C-terminal region. Reversion to PVY N -like sequence was also revealed in the NIa N-terminal area of PVY NTN isolates, followed by a switching back to a PVY O -like sequence in the CP gene. Lastly, some PVY N W isolates showed a switching from PVY O - to PVY N -like sequence in the P1 N-terminal part, thus separating our PVY N W isolates into two subgroups. All these apparent recombination events were shown by statistical analysis. Comparison of molecular traits with pathogenic properties of our isolates suggested that the HC-Pro protein is involved in induction of necrosis in tobacco leaves, and the NIa, NIb and/or CP protein in necrosis in potato tubers. Nevertheless, multiple recombination events observed in the PVY NTN genome may play a role in the latter phenomenon. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Genomic variability in Potato potyvirus Y (PVY): evidence that PVY N W and PVY NTN variants are single to multiple recombinants between PVY O and PVY N isolates

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Publisher
Springer-Verlag
Copyright
Copyright © 2002 by Springer-Verlag/Wien
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s705-002-8325-0
Publisher site
See Article on Publisher Site

Abstract

Fourteen Potato virus Y (PVY) isolates representative of PVY O , PVY N , PVY NTN and PVY N W groups were characterised at genomic level. Restriction fragment length polymorphism study (RFLP) of each gene of these isolates and sequencing of the first 2700 nucleotides of two PVY N W isolates were performed. A mosaic structure was revealed in PVY N W and PVY NTN genomes, which showed either PVY O or PVY N -like sequences, depending on the particular gene. Indeed, starting from the 5′-end, these isolates showed a switching, from PVY N - to PVY O -like sequence, in the HC-Pro C-terminal region. Reversion to PVY N -like sequence was also revealed in the NIa N-terminal area of PVY NTN isolates, followed by a switching back to a PVY O -like sequence in the CP gene. Lastly, some PVY N W isolates showed a switching from PVY O - to PVY N -like sequence in the P1 N-terminal part, thus separating our PVY N W isolates into two subgroups. All these apparent recombination events were shown by statistical analysis. Comparison of molecular traits with pathogenic properties of our isolates suggested that the HC-Pro protein is involved in induction of necrosis in tobacco leaves, and the NIa, NIb and/or CP protein in necrosis in potato tubers. Nevertheless, multiple recombination events observed in the PVY NTN genome may play a role in the latter phenomenon.

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2002

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